PSMA7 Rabbit pAb - O14818

Core Product Specifications and Parameters

Biological Background: PSMA7 Function and Localization

• PSMA7 encodes proteasome subunit alpha type-7, also known as proteasome subunit RC6-1, XAPC7, or alpha-4. UniProt: O14818.
• Core component of the 20S proteasome, a barrel-shaped complex that performs proteolytic degradation of intracellular proteins. Associates with regulatory particles to form 26S proteasome (ATP-dependent ubiquitinated protein degradation), or with PA200/PA28 for ubiquitin-independent degradation (e.g., spermatogenesis, MHC class I antigen processing).
• Plays roles beyond proteolysis: inhibits HIF-1A transactivation under normoxic and hypoxic conditions; interaction with EMAP2 enhances HIF-1A degradation.
• Mediates hepatitis C virus internal ribosome entry site (IRES)-mediated translation and promotes nuclear translocation of androgen receptor, enhancing androgen-mediated transactivation.
• Negatively regulates innate immune response by promoting MAVS degradation.
• Localizes to both cytoplasm and nucleus.
• Post-translational modifications include acetylation, phosphorylation, and glycosylation; alternative splicing produces isoforms.
• Molecular weight: approximately 28 kDa (calculated 27.9 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human PSMA7 (residues 1–248), which covers the entire alpha-4 subunit. Expect detection of the ~28 kDa band in western blot.
• For IHC-P and IF/ICC, note that PSMA7 localizes to both cytoplasm and nucleus; ensure appropriate fixation and permeabilization conditions for nuclear antigens.
• Cross-reactivity with mouse and rat has been reported; verify in your specific sample matrix.
• Suitable for ELISA applications; consider using with recombinant PSMA7 or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical support, and consulting—from concept to clinic. This PSMA7 antibody supports proteasome and protein degradation studies, and we can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your assay development.