PRSS2 Rabbit Polyclonal Antibody for WB and ELISA - P07478

Core Product Specifications and Parameters

Biological Background: PRSS2 Function and Localization

• PRSS2 encodes trypsin-2, also known as anionic trypsinogen, serine protease 2, and trypsin II.
• It is a secreted serine protease that in the ileum may be involved in defensin processing, including DEFA5.
• The protein localizes to the extracellular space upon secretion.
• PRSS2 is specifically expressed in Paneth cells, which reside at the base of small intestinal crypts.
• Synthesized as a zymogen, trypsin-2 requires proteolytic activation and calcium binding for full activity.
• As a member of the serine protease family, it participates in digestive processes and general proteolysis.
• The mature protein has a molecular weight of approximately 26.5 kDa, consistent with the 26 kDa band observed by SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 24–247 of human PRSS2, covering most of the mature protein, which may allow detection of both the pro-form and active enzyme.
• For Western blot, a band around 26 kDa is expected; validate in human and mouse intestinal tissue lysates or Paneth cell-derived lines.
• For ELISA, the antibody can be used for direct antigen detection; consider optimizing coating conditions and, if developing a sandwich assay, pairing with a capture antibody.
• Because PRSS2 is secreted, biological fluids (serum, intestinal lavage) or cell culture supernatants may be suitable sample types, though matrix effects should be assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering this anti-PRSS2 polyclonal antibody alongside a wide range of digestive enzyme and protease-related reagents. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and customized monoclonal antibody services to streamline your diagnostic development. Whether you need established tools or rare-target sourcing, CamelBio supports your project from concept to clinical application.