PRRT2 Rabbit pAb for WB and ELISA - Q7Z6L0

Core Product Specifications and Parameters

Biological Background: PRRT2 Function and Localization

• PRRT2 encodes proline-rich transmembrane protein 2, also known as dispanin subfamily B member 3 (DSPB3).
• It functions in the central nervous system as a component of the AMPA receptor (AMPAR) complex, contributing to synaptic transmission.
• In hippocampal neurons, PRRT2 localizes to presynaptic terminals and regulates neurotransmitter release, potentially by modulating calcium sensing.
• In the cerebellum, PRRT2 inhibits SNARE complex formation and downregulates short-term synaptic facilitation.
• Subcellularly, PRRT2 is found at the cell membrane, presynaptic membrane, postsynaptic density, and on synaptic vesicles and dendritic spines.
• It undergoes alternative splicing and is subject to post-translational modifications including methylation and phosphorylation.
• Mutations in PRRT2 are associated with neurological disorders such as epilepsy and dystonia, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 20-130 of human PRRT2; this N-terminal region is conserved in mouse, supporting cross-reactivity. Optimize blocking and washing conditions for your specific samples.
• For Western blot, expect a band at approximately 35 kDa. Validate in neuronal or brain lysates, and consider using positive controls expressing PRRT2.
• In ELISA, titrate the antibody to determine optimal coating and detection conditions; the polyclonal nature allows recognition of multiple epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your PRRT2-related neuroscience research and diagnostic development with a comprehensive portfolio of validated antibody pairs, recombinant proteins, and ancillary IVD raw materials. We offer scalable sourcing for rare targets like PRRT2, including bulk polyclonal antibodies and optimized reagents, to accelerate your projects from concept to clinic.