PRPF8 Rabbit pAb - Q6P2Q9

Core Product Specifications and Parameters

Biological Background: PRPF8 Function and Localization

• Pre-mRNA-processing-splicing factor 8 (PRPF8), also termed PRP8 homolog, 220 kDa U5 snRNP-specific protein, or p220, is an essential spliceosomal component.
• Serves as a core factor in precatalytic, catalytic, and postcatalytic spliceosomal complexes for both major U2-type and minor U12-type splicing pathways. Related references: PMID:10411133 PMID:11971955 PMID:28076346
• Acts as a molecular scaffold that orchestrates the ordered assembly of spliceosomal proteins and snRNAs, and is crucial for formation of the U4/U6-U5 tri-snRNP complex.
• Positions U2, U5, and U6 snRNAs at the splice sites on pre-mRNA, ensuring precise splicing catalysis.
• Directly interacts with both the 5' and 3' splice site regions, contributing to splice site recognition.
• Resides in the nucleus and nuclear speckles, consistent with its central role in mRNA processing.
• Ubiquitously expressed across human tissues, highlighting its fundamental housekeeping function.
• Subject to post-translational modifications including acetylation, methylation, and phosphorylation; mutations in PRPF8 are associated with retinitis pigmentosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100), which may yield antibodies recognizing the N-terminus of PRPF8.
• For Western blot, a band of approximately 274 kDa may be detected; consider using low-percentage gels and extended transfer times for high-molecular-weight proteins.
• When performing immunohistochemistry (IHC-P), optimization of antigen retrieval methods is recommended to achieve robust staining.
• In immunofluorescence/ICC, a nuclear and speckled staining pattern is anticipated, based on the known localization of PRPF8.
• As a polyclonal antibody, lot-to-lot variability is possible; validate in your specific assay system and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a specialized one-stop provider of IVD raw materials, streamlining procurement for diagnostic manufacturers and research institutions. This PRPF8 antibody supports investigations into spliceosome dynamics and RNA processing, with potential integration into diagnostic workflows. We offer tailored solutions including validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and assistance in sourcing rare targets—helping you accelerate development from concept to clinic.