PRPF4 Rabbit mAb - O43172

Core Product Specifications and Parameters

Biological Background: PRPF4 Function and Localization

• PRPF4 encodes the U4/U6 small nuclear ribonucleoprotein Prp4, also known as hPrp4 or U4/U6 snRNP 60 kDa protein, a core component of the spliceosome.
• As part of the U4/U6-U5 tri-snRNP complex, PRPF4 is essential for pre-mRNA splicing; it participates in spliceosome assembly and is present in the precatalytic B complex.
• PRPF4 belongs to the WD repeat protein family, characterized by multiple WD40 repeats that mediate protein-protein interactions.
• The protein localizes to the nucleus and is enriched in nuclear speckles, which are storage and modification sites for splicing factors.
• PRPF4 undergoes post-translational acetylation, which may regulate its activity or interactions.
• Mutations in PRPF4 are associated with retinitis pigmentosa, an inherited retinal degenerative disease, highlighting its importance beyond splicing.
• Alternative splicing of PRPF4 produces multiple isoforms, potentially expanding its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (aa 450-522) of human PRPF4, a domain likely to be conserved across species; consider sequence alignment to predict cross-reactivity in other organisms.
• For Western blot (WB), the predicted molecular weight of PRPF4 is ~58 kDa; verify using appropriate positive controls (e.g., HeLa or HEK293 cell lysates) and use fresh protease inhibitors to prevent degradation.
• In immunohistochemistry (IHC-P), nuclear staining is expected, with possible nuclear speckle enrichment; optimize antigen retrieval and antibody dilution for paraffin-embedded sections.
• For ELISA, the antibody may be used to detect recombinant or endogenous PRPF4; validate linearity and specificity in the relevant sample matrix.
• Since PRPF4 is involved in spliceosome assembly, consider testing co-immunoprecipitation to study protein interactions, though this application is not validated for this antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this PRPF4 Rabbit mAb, supporting spliceosome-related studies and diagnostic development. Beyond antibodies, we supply bulk ancillary reagents and offer rare target sourcing to streamline your workflow from concept to clinic. Our one-stop platform simplifies procurement, enabling you to focus on advancing your splicing biology research or diagnostic applications.