PROZ Rabbit pAb - P22891

Core Product Specifications and Parameters

Biological Background: PROZ Function and Localization

• Protein Z (PZ) is a vitamin K-dependent plasma glycoprotein that serves as a cofactor in the regulation of blood coagulation.
• It binds thrombin and facilitates its association with phospholipid vesicles, contributing to the inhibition of factor Xa in the presence of the serpin SERPINA10, calcium, and phospholipids.
• As a member of the serine protease homolog family containing an EGF-like domain and gamma-carboxyglutamic acid residues, PROZ is structurally related to other vitamin K-dependent coagulation factors.
• The protein is secreted into the blood plasma.
• Post-translational modifications include gamma-carboxyglutamic acid formation, hydroxylation, and disulfide bond formation, which are critical for its activity.
• Alternative splicing generates multiple isoforms, though the full-length secreted form is predominant.
• Keywords associated with PROZ include blood coagulation, hemostasis, secreted, glycoprotein, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 220-340 of human PROZ, a region that may provide specificity for the full-length and relevant isoforms. When designing experiments, consider verifying reactivity with endogenous protein in relevant samples.
• This polyclonal antibody has been validated for use in Western blot (WB) and ELISA. For species reactivity, human, mouse, and rat are confirmed; however, cross-reactivity with other species should be tested individually.
• Given the secreted nature of PROZ, cell lysates may not be optimal; consider using plasma or serum samples for detection, and include positive controls.
• The predicted molecular weight is approximately 45 kDa, but glycosylation and other modifications may cause migration shifts in SDS-PAGE.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects targeting coagulation and hemostasis biomarkers like protein Z, we can assist in sourcing rare-target raw materials and offer technical consulting from concept to clinic.