PRODH Rabbit pAb for WB and ELISA - O43272

Core Product Specifications and Parameters

Biological Background: Proline Dehydrogenase 1 Function and Localization

• Encoded by the PRODH gene (also known as PIG6, POX2, PRODH2), proline dehydrogenase 1 (PRODH) is a mitochondrial enzyme responsible for catalyzing the conversion of proline to delta-1-pyrroline-5-carboxylate, the first and rate-limiting step in proline catabolism.
• PRODH is a member of the proline dehydrogenase family and functions as a FAD-dependent oxidoreductase, utilizing flavin adenine dinucleotide as a cofactor.
• The protein localizes to the mitochondrial matrix, where it participates in proline metabolism and links amino acid degradation to cellular energy production and apoptosis.
• Expression is highest in lung, skeletal muscle, and brain tissues, with moderate levels in heart and kidney, and low levels in liver, placenta, and pancreas.
• Alternative splicing generates multiple isoforms that may be regulated in a tissue-specific manner.
• Post-translational modifications include acetylation, which may modulate enzyme activity or stability.
• Genetic variations and mutations in PRODH are associated with hyperprolinemia type I and have been implicated in schizophrenia susceptibility, highlighting its relevance to neuropsychiatric disorders.
• As a p53-induced gene (PIG6), PRODH is involved in p53-mediated pro-apoptotic pathways, linking proline metabolism to tumor suppression.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 321–600 of human PRODH (NP_057419.4), a region predicted to be conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• In Western blotting, the antibody is expected to detect a band near 68 kDa (UniProt calculated mass 68 kDa). Minor bands reflecting isoforms or post-translational modifications may appear depending on sample preparation.
• For ELISA, the polyclonal format provides high avidity suitable for capture or detection; consider pairing with a monoclonal detector or using indirect ELISA for quantification.
• Validate the antibody in a relevant biological context (e.g., lung or brain tissue lysates) to confirm specificity, as PRODH expression varies by tissue.
• When probing cancer cells, induction by p53 (e.g., via doxorubicin treatment) may upregulate PRODH, serving as a positive control for target modulation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a streamlined single-source solution for IVD raw materials. This anti-PRODH antibody supports investigations into proline metabolism, mitochondrial function, and cancer biology. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonals, and bulk ancillary reagents, alongside custom procurement of rare targets—ensuring you have the critical materials needed from concept to clinic.