PRKAR1A Rabbit Polyclonal Antibody (KO Validated) for WB, IF/ICC, ELISA - P10644

Core Product Specifications and Parameters

Biological Background: PRKAR1A Function and Localization

• Encodes the regulatory subunit I-alpha of cAMP-dependent protein kinase (PKA), which mediates cAMP signaling in cells.
• Functions as a key component of the PKA holoenzyme; binding of cAMP to the regulatory subunit releases the catalytic subunits, activating downstream phosphorylation cascades.
• Also known as Tissue-specific extinguisher 1 (TSE1), reflecting its role in tissue-specific gene regulation.
• Localizes to the cell membrane, positioning PKA near membrane-bound receptors and channels for rapid signal transduction.
• Contains two cAMP-binding domains and is subject to phosphorylation and acetylation, modulating its activity and interactions.
• Belongs to a family of four regulatory subunit types (I-alpha, I-beta, II-alpha, II-beta) with tissue-specific and inducible expression patterns.
• Mutations are associated with Cushing syndrome and other endocrine tumors, highlighting its role in hormone signaling and cell growth.
• Alternative splicing generates multiple isoforms, potentially diversifying its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-250 of human PRKAR1A; this region includes the N-terminal dimerization/docking domain but not the cAMP-binding domains. For applications requiring detection of endogenous full-length protein, WB under reducing conditions is recommended.
• This antibody is suitable for WB, IF/ICC, and ELISA based on supplier specifications. For IF/ICC, permeabilization may be necessary to access the intracellular/membrane-associated target.
• Predicted to cross-react with human, mouse, and rat. Consider sequence homology of the immunogen region across species to anticipate reactivity in other models.
• Validate antibody performance in your specific sample type and application, as conditions may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, technical services, and consultancy. This PRKAR1A polyclonal antibody supports research in cAMP signaling and protein kinase pathways, offering a reliable tool for studying hormone-related disorders and cancer biology. Beyond individual antibodies, we supply validated antibody pairs, monoclonal/polyclonal options, bulk ancillary reagents, and rare target sourcing—streamlining your development from concept to clinic.