PPP1CC Rabbit Polyclonal Antibody for WB and ELISA - P36873

Core Product Specifications and Parameters

Biological Background: PPP1CC Function and Localization

• PPP1CC is the catalytic subunit of protein phosphatase 1 (PP1), which associates with over 200 regulatory subunits to form holoenzymes that dephosphorylate hundreds of cellular targets, including RPS6KB1. Related references: PMID:17936702, PMID:25012651
• PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis.
• As part of the PTW/PP1 phosphatase complex, PPP1CC controls chromatin structure and cell cycle progression during the transition from mitosis into interphase. Related references: PMID:20516061
• In balance with casein kinase 1 delta/epsilon (CSNK1D/E), PPP1CC determines circadian period length by regulating the phosphorylation speed and rhythmicity of PER1 and PER2; it may also dephosphorylate CSNK1D/E. Related references: PMID:21712997
• PPP1CC regulates kinetochore function by controlling SKA complex recruitment and dephosphorylates MKI67 at the onset of anaphase. Related references: PMID:28982702, PMID:25012651
• In immune regulation, PPP1CC dephosphorylates FOXP3 in regulatory T cells from rheumatoid arthritis patients, inactivating FOXP3 and impairing Treg function; together with PPP1CA, it dephosphorylates and activates IFIH1/MDA5 and RIG-I, promoting innate immune responses. Related references: PMID:23396208, PMID:23499489
• As a core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex, PPP1CC dephosphorylates and stimulates RAF kinases (RAF1, BRAF, ARAF), thereby activating the MAPK signaling pathway. Related references: PMID:35768504, PMID:35831509
• Subcellular localization includes cytoplasm, nucleus, nucleolus, nucleoplasm, nuclear speckles, centromeres, kinetochores, cleavage furrow, midbody, mitochondria, and the microtubule organizing center.
• UniProt keywords indicate that PPP1CC is a phosphoprotein, acetylated, and subject to alternative splicing; a disease variant has been reported for this gene.

Experimental Guidance and Technical Tips

• The immunogen (full-length recombinant protein, aa 1–323) is expected to generate a polyclonal antibody recognizing multiple epitopes; consider this broad reactivity when planning applications such as ELISA that may require native conformation.
• For Western blot, a band at approximately 37 kDa is anticipated; validate detection in human samples, as cross‑reactivity is reported only for human.
• Given PPP1CC’s involvement in multiple signaling pathways, select appropriate positive and negative controls based on known expression levels in relevant cell lines or tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-PPP1CC polyclonal antibody as part of a comprehensive portfolio for cell cycle, circadian rhythm, and signaling pathway research. Our one-stop sourcing platform provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and access to rare target raw materials, supporting your IVD development from concept to clinic.