PPM1G Rabbit mAb for WB and ELISA - O15355

Core Product Specifications and Parameters

Biological Background: PPM1G Function and Localization

• PPM1G, also designated protein phosphatase 1G, protein phosphatase 1C, protein phosphatase 2C isoform gamma (PP2C-gamma), or protein phosphatase magnesium-dependent 1 gamma, belongs to the PP2C family of serine/threonine phosphatases.
• It functions as a metal-dependent protein phosphatase that requires magnesium or manganese ions for catalytic activity, as indicated by its classification as a magnesium-dependent phosphatase (PPM).
• Subcellular localization includes the cytoplasm and membrane, suggesting its involvement in membrane-associated signaling events.
• The protein is widely expressed, with highest abundance in testis, skeletal muscle, and heart, pointing to roles in development, muscle function, and cardiac physiology.
• Post-translational modifications include acetylation, methylation, phosphorylation, and lipidation (myristoylation), which may regulate its activity, localization, or interactions.
• The UniProtKB entry categorizes PPM1G under hydrolase, lipoprotein, and reference proteome, highlighting its catalytic function and comprehensive sequence characterization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 301-450 of human PPM1G, spanning the C-terminal region. This recombinant protein design suggests the antibody may recognize full-length PPM1G and possibly C-terminal fragments.
• For Western blotting (WB), expect a band at approximately 59kDa. Validate specificity by using appropriate positive (e.g., HeLa, HEK293) and negative controls.
• In ELISA, consider optimizing the antibody concentration for both direct and sandwich formats; pairing with a detection antibody against a different epitope may enhance sensitivity.
• As a rabbit monoclonal antibody, it offers high batch-to-batch consistency. Confirm cross-reactivity with human samples and test any potential reactivity in other species if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, streamlining procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target materials. This PPM1G antibody supports research into phosphatase-mediated signaling pathways, with one-stop access to technical support and supply chain solutions—from concept to clinic—for diagnostic manufacturers and laboratories.