PPIL4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WUA2

Core Product Specifications and Parameters

Biological Background: Peptidyl-prolyl cis-trans isomerase-like 4 Function and Localization

• PPIL4, also designated Cyclophilin-like protein PPIL4 or Rotamase PPIL4, belongs to the peptidyl-prolyl cis-trans isomerase (PPIase) family and shares structural features with cyclophilins.
• It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, a critical rate-limiting step in protein folding (By similarity).
• PPIL4 is localized predominantly in the nucleus, implying a role in nuclear protein maturation or regulatory processes.
• Among adult human tissues, PPIL4 is abundantly expressed in the kidney, while other tissues exhibit ubiquitously low expression levels.
• The protein contains potential phosphosites, suggesting regulation by phosphorylation, and is a target for ubiquitin-like modifications as indicated by its isopeptide bond formation and Ubl conjugation.
• PPIL4 has been reported to possess RNA-binding capacity, possibly linking it to post-transcriptional control of gene expression.
• Its three-dimensional structure has been determined, providing insights into its enzymatic mechanism.
• As a rotamase, PPIL4 facilitates the rotation of peptide bonds, influencing protein conformation and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 301-492 of human PPIL4; verify cross-reactivity if using samples from other species.
• For Western blot, a band at approximately 57 kDa is anticipated; validate with appropriate positive control lysates, such as kidney tissue extracts.
• In immunofluorescence/ICC, expect predominantly nuclear staining; include nuclear counterstains to confirm localization.
• As a polyclonal antibody, batch-to-batch variability may occur; consider titration and validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise spans rare target sourcing, including peptidyl-prolyl isomerases like PPIL4, supporting your nuclear protein and folding research. From concept to clinic, CamelBio streamlines your diagnostic development with reliable, scalable supply solutions.