PPIL1 Rabbit pAb - Q9Y3C6

Core Product Specifications and Parameters

Biological Background: PPIL1 Function and Localization

• PPIL1 (peptidyl-prolyl cis-trans isomerase-like 1), also designated rotamase PPIL1, catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Related references: PMID:16595688
• As a component of the spliceosome, PPIL1 participates in pre-mRNA splicing. Related references: PMID:11991638, PMID:28076346, PMID:28502770
• PPIL1 catalyzes prolyl peptide bond isomerization in CDC40/PRP17, a splicing factor. Related references: PMID:33220177
• The protein plays an essential role in embryonic brain development, a function that is independent of its isomerase activity. Related references: PMID:33220177
• Subcellular localization is predominantly nuclear.
• PPIL1 is ubiquitously expressed, with highest levels observed in heart and skeletal muscle.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword); disease variants are associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers the N-terminal region (aa 1-166) of human PPIL1, which may ensure recognition of endogenous protein across human, mouse, and rat samples.
• For Western blot, consider using whole-cell lysates from heart or skeletal muscle tissues where PPIL1 expression is highest; verify a band near 18 kDa.
• For ELISA, titrate the antibody to determine the optimal working concentration and validate specificity using recombinant PPIL1 protein or peptide blocking.
• Because PPIL1 functions in the spliceosome, consider testing the antibody in nuclear extracts or splicing-competent cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge, providing diagnostic manufacturers and research laboratories with bulk IVD raw materials and technical support—from concept to clinic. This PPIL1 Rabbit pAb meets the needs for studying pre-mRNA splicing and isomerase activity, and it is backed by our supply capabilities including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. Whether you require rare-target antibodies or large-scale production, CamelBio is your reliable partner in advancing diagnostic and research applications.