PPA2 Rabbit pAb for WB, IF/ICC, ELISA - Q9H2U2

Core Product Specifications and Parameters

Biological Background: PPA2 Function and Localization

• Hydrolyzes inorganic pyrophosphate (PPi), providing phosphate for various metabolic processes. Related references: PMID:27523597
• Essential for the regulation of mitochondrial membrane potential, mitochondrial organization, and function. Related references: PMID:27523598
• Localizes to the mitochondrion, highlighting its role in mitochondrial bioenergetics.
• Detected in multiple tissues including brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood, and kidney proximal tubule cells.
• Post-translational modifications include acetylation and phosphorylation, potentially modulating activity.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• Associated with metabolic diseases; classified as a hydrolase requiring magnesium and metal binding for activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal region (amino acids 33–100) of human PPA2, potentially recognizing full-length and some processed forms. Consider validating in mitochondrial lysates.
• For Western blotting, the predicted band size is ~38 kDa; optimize gel percentage and transfer conditions accordingly.
• In immunofluorescence, confirm mitochondrial localization by co-staining with mitochondrial markers (e.g., MitoTracker or anti-TOM20).
• Endogenous expression in human, mouse, and rat cell lines or tissues with high mitochondrial content (e.g., skeletal muscle, kidney) can serve as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For mitochondrial research targets like PPA2, we provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Contact us to secure high-quality PPA2 antibodies and explore rare target sourcing for your bioenergetics studies.