PP2A alpha/beta Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - P67775 / P62714

Core Product Specifications and Parameters

Biological Background: PP2A alpha/beta Function and Localization

• Encoded by PPP2CA (GeneID 5515) and PPP2CB (GeneID 5516), PP2A catalytic subunit alpha/beta is a highly conserved serine/threonine phosphatase. This antibody recognizes both isoforms (SWISS: P67775/P62714), which share significant homology.
• PP2A functions as the major phosphatase for microtubule-associated proteins and modulates key kinases (e.g., phosphorylase B kinase, casein kinase 2). It dephosphorylates diverse substrates such as p53, MYC, and AKT1, influencing cell cycle progression, apoptosis, and signal transduction (PubMed:10801873, PubMed:12473674, PubMed:17245430, PubMed:22613722, PubMed:25438055, PubMed:30513302). Related references: PMID:10801873 PMID:12473674 PMID:17245430 PMID:22613722 PMID:25438055 PMID:30513302
• Participates in the STRIPAK complex, regulating Hippo and MAPK pathways critical for cell growth and differentiation (PubMed:33633399). Related references: PMID:33633399
• Within the INTAC complex, PPP2CA dephosphorylates the RNA polymerase II C-terminal domain, mediating transcription termination at quality checkpoints (PubMed:33243860, PubMed:34004147, PubMed:37080207). Related references: PMID:33243860 PMID:34004147 PMID:37080207
• Subcellular distribution includes cytoplasm, nucleus, centromeres, and spindle poles, consistent with its roles in mitosis, meiosis, and transcription.
• Post-translational modifications, such as methylation at the C-terminal tail and phosphorylation, regulate its activity and interactions. Disease-associated variants are linked to intellectual disability.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody is raised against a synthetic peptide within amino acids 200-309 of human PP2A alpha and beta (based on P62714). It is predicted to detect both isoforms.
• For Western blot (WB), a band corresponding to ~36 kDa should be observed. Use appropriate positive controls (e.g., HeLa, Jurkat cell lysates) and validate in your specific sample system.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval methods and tissue fixation conditions, as PP2A localization may vary.
• For ELISA, optimize coating antigen and antibody dilution pairs. Cross-reactivity with human, mouse, and rat has been reported by the supplier; verify species-specific reactivity in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This PP2A alpha/beta antibody supports phosphatase research with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Contact us for rare-target sourcing and tailored solutions in signal transduction and cancer research.