POLR2I Rabbit pAb - P36954

Core Product Specifications and Parameters

Biological Background: DNA-directed RNA Polymerase II Subunit RPB9 (POLR2I) Function and Localization

• The POLR2I gene encodes the DNA-directed RNA polymerase II subunit RPB9, also known as DNA-directed RNA polymerase II subunit I, RPB14.5, or hRPB14.5. It is a 14.5 kDa essential component of the core Pol II transcription machinery.
• As a core subunit of RNA polymerase II, POLR2I/RPB9 participates in the synthesis of all mRNA precursors and many functional non-coding RNAs, driving transcription initiation, elongation, and termination.
• RPB9 resides within the Pol II "upper jaw" domain, which is thought to grip the incoming DNA template and contribute to transcription fidelity and processivity.
• The protein localizes predominantly to the nucleus and nucleolus, consistent with its fundamental role in transcription.
• POLR2I is annotated with a zinc-finger domain and metal-binding capacity; post-translational modifications such as acetylation have been identified, though their functional significance requires further elucidation.

Experimental Guidance and Technical Tips

• For Western blot detection, nuclear extracts or whole-cell lysates from human or mouse cell lines are recommended; a specific band near 15 kDa (predicted 14.5 kDa) consistent with endogenous POLR2I is expected. Optimize sample loading and transfer conditions to resolve low-molecular-weight proteins.
• The immunogen comprises amino acids 1–125 of human POLR2I (NP_006224.1), covering nearly the full-length protein. This design likely enables recognition of both native and denatured forms; validate performance in your relevant sample system.
• For ELISA applications, consider coating with recombinant POLR2I protein or peptide antigens. Pair with appropriate secondary antibodies and optimize blocking and wash steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by offering a comprehensive portfolio of IVD raw materials, including validated monoclonal/polyclonal antibodies, antibody pairs, and ancillary reagents. This POLR2I rabbit polyclonal antibody targets a critical component of the RNA polymerase II transcription machinery, making it a valuable tool for transcription research and potential IVD assay development. Whether you need bulk antibody supplies, rare-target sourcing, or integrated technical consulting from concept to clinic, CamelBio serves as your dedicated sourcing partner.