POLR2E Rabbit pAb - P19388

Core Product Specifications and Parameters

Biological Background: POLR2E Function and Localization

• POLR2E encodes the DNA-directed RNA polymerases I, II, and III subunit RPABC1, a common component of all three nuclear RNA polymerases that is essential for transcription of ribosomal RNA, messenger RNA, and small RNA species.
• The protein, also known as RPB5 or XAP4, is a 25 kDa subunit that forms part of the RNA polymerase II lower jaw domain, where it is thought to help grip the incoming DNA template during transcription.
• As a shared subunit, RPABC1 participates in the synthesis of rRNA precursors by Pol I, mRNA precursors and non‑coding RNAs by Pol II, and small RNAs like 5S rRNA and tRNAs by Pol III.
• Subcellularly, POLR2E localizes predominantly to the nucleus and is enriched in the nucleolus, consistent with its role in ribosomal RNA transcription.
• Post‑translational modifications include acetylation and ubiquitination (isopeptide bond), which may regulate polymerase complex assembly, stability, or activity.
• The protein is also implicated in host‑virus interactions, as annotated by the UniProt keyword.

Experimental Guidance and Technical Tips

• The immunogen used covers the full-length human POLR2E protein (amino acids 1–210), suitable for detecting total POLR2E levels in applications such as Western blot (predicted band ~25 kDa) and immunofluorescence.
• Given the high sequence conservation among human, mouse, and rat POLR2E, the antibody can be used for cross‑species studies, but validation in each system is advisable.
• For immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), optimize antigen retrieval and permeabilization conditions to enhance nuclear/nucleolar signal.
• As a polyclonal antibody, lot‑to‑lot consistency should be monitored, and alternative detection reagents (e.g., monoclonal antibodies) may be considered for long‑term studies requiring high reproducibility.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is a dedicated supplier of high‑quality IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from discovery to clinical application. This POLR2E antibody, targeting a conserved transcription machinery subunit, complements our portfolio of validated antibodies for gene expression and oncology research. Beyond individual reagents, CamelBio offers a full range of services including matched antibody pairs, bulk ancillary reagents, and custom sourcing of rare‑target raw materials to streamline your assay development.