POLR2A Rabbit pAb for WB, IF/ICC, IHC-P, ELISA, ChIP - P24928

Core Product Specifications and Parameters

Biological Background: POLR2A Function and Localization

• DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is the largest and catalytic subunit of RNA polymerase II, essential for transcribing protein-coding genes and numerous non-coding RNAs. Related references: PMID:23748380 PMID:27193682 PMID:30190596 PMID:28108474
• The polymerase catalyzes the template-directed addition of ribonucleotides, with its active site coordinated by magnesium ions; it also possesses a 3'→5' exonuclease proofreading activity. Related references: PMID:8381534 PMID:9852112
• A unique C-terminal domain (CTD) containing 52 heptapeptide repeats acts as a regulatory hub, with phosphorylation at Ser5 facilitating promoter escape and Ser2 promoting elongation and pre-mRNA processing. Related references: PMID:24207025 PMID:26124092
• Beyond DNA-dependent transcription, POLR2A can function as an RNA-dependent RNA polymerase, extending non-coding RNAs using an internal template, potentially influencing gene silencing.
• Localized primarily in the nucleus, with presence in the cytoplasm and on chromosomes; post-translational modifications include acetylation, methylation, ubiquitination, and phosphorylation.
• Mutations in POLR2A are associated with neurodevelopmental disorders, and the protein is targeted by viruses such as Hepatitis delta virus for replication of its genome.

Experimental Guidance and Technical Tips

• For Western blot, due to the large protein size (217 kDa), use a 6-8% polyacrylamide gel and ensure efficient transfer (e.g., wet transfer with 10% methanol). A positive control such as HeLa or HEK293 cell lysate may be considered.
• For IF/ICC and IHC-P, fixation and antigen retrieval conditions should be optimized; permeabilization with Triton X-100 may be necessary for intracellular nuclear targets.
• For ChIP, the N-terminal epitope location suggests suitability for detecting native POLR2A, but validation with positive and negative target genes is recommended.
• As a polyclonal antibody, lot-to-lot consistency is expected but should be verified in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a curated portfolio of IVD-grade antibodies, including the POLR2A rabbit polyclonal antibody, optimized for applications such as Western blot, immunofluorescence, and chromatin immunoprecipitation. As a dedicated sourcing partner for diagnostic manufacturers, we supply validated raw materials and can assist with custom requirements, from bulk antibody production to ancillary reagents, accelerating your assay development from concept to clinic.