PNPLA3 Rabbit Polyclonal Antibody (Adiponutrin, iPLA2-epsilon) for WB and ELISA - Q9NST1

Core Product Specifications and Parameters

Biological Background: PNPLA3 Function and Localization

• PNPLA3 catalyzes the CoA-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (LPA) to generate phosphatidic acid (PA), a critical precursor in triglyceride and phospholipid synthesis. It preferentially utilizes long-chain fatty acyl-CoAs (≥C16) such as arachidonoyl-CoA and oleoyl-CoA (PubMed:22560221). Related references: PMID:22560221
• The enzyme also exhibits low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities, suggesting a role in triglyceride acyl-chain remodeling (PubMed:15364929, PubMed:20034933, PubMed:22560221). Related references: PMID:15364929, PMID:20034933, PMID:22560221
• In vitro, PNPLA3 displays hydrolytic activity toward tri-, di-, and monoacylglycerols with a preference for oleic acid, though its triacylglycerol hydrolase activity is controversial and may be very low (PubMed:21878620, PubMed:22560221). Related references: PMID:21878620, PMID:22560221
• The protein possesses phospholipase A2 activity, further indicating its multifunctional nature in lipid metabolism (PubMed:15364929). Related references: PMID:15364929
• PNPLA3 localizes to lipid droplets and cellular membranes, consistent with its roles in lipid storage and membrane phospholipid biosynthesis.
• Genetic variants of PNPLA3, notably the I148M substitution, are strongly associated with obesity, non-alcoholic fatty liver disease (NAFLD), and progression to steatohepatitis, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 377–481) targets the C-terminal region; consider this when designing assays or detecting isoforms/cleavage products.
• The predicted molecular weight is ~53 kDa; however, glycosylation and other modifications may cause band shifts in Western blot, so use appropriate positive controls such as human liver or HepG2 lysates.
• For ELISA, optimize coating buffer and detection antibody concentrations using recombinant PNPLA3 as a standard.
• Cross-reactivity with mouse and rat is indicated; validate by comparing sequences in the immunogen region to ensure epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing bridge for IVD raw materials, supporting every stage from concept to clinic. This anti-PNPLA3 polyclonal antibody facilitates research into lipid metabolism disorders like NAFLD. We offer validated antibody pairs, bulk ancillary reagents, and can assist in sourcing rare targets to accelerate your diagnostic development.