PNMT Rabbit pAb - P11086

Core Product Specifications and Parameters

Biological Background: Phenylethanolamine N-methyltransferase Function and Localization

• Catalyzes the transmethylation of norepinephrine to form epinephrine, a key step in catecholamine biosynthesis, using S-adenosyl-L-methionine as the methyl donor (PubMed:20496117). Related references: PMID:20496117
• Also methylates other substrates including phenylethanolamine, octopamine, and normetanephrine (PubMed:16277617, PubMed:16363801, PubMed:8812853; By similarity). Related references: PMID:16277617, PMID:16363801, PMID:8812853
• Alternative name: Noradrenaline N-methyltransferase.
• Belongs to the methyltransferase superfamily; S-adenosyl-L-methionine-dependent transferase.
• Reported as a phosphoprotein; post-translational modifications may regulate its activity.
• Predicted molecular weight approximately 30.9 kDa (UniProt).

Experimental Guidance and Technical Tips

• For western blotting, denaturing SDS-PAGE conditions are recommended; the target protein migrates at around 31 kDa; validate in your sample matrix.
• In ELISA, this polyclonal antibody can be used for capture or detection; consider affinity purification for higher specificity.
• The antibody is raised against full-length recombinant PNMT (aa 1-282); it may recognize both phosphorylated and unphosphorylated forms; confirm with appropriate controls.
• Cross-reactivity has been observed with human and mouse samples; for other species, in-house validation is advised.
• Shipment on ice bags; store at -20°C upon arrival, avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials, covering every stage from concept to clinic. For targets such as PNMT, involved in catecholamine biosynthesis, we supply premium polyclonal antibodies together with validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development.