PMS2 Rabbit Monoclonal Antibody for WB, ELISA - P54278

Core Product Specifications and Parameters

Biological Background: PMS2 Function and Localization

• PMS2 (Postmeiotic Segregation Increased 2) is a core component of the post-replicative DNA mismatch repair (MMR) system, crucial for maintaining genomic fidelity (PubMed:30653781, PubMed:35189042). Related references: PMID:30653781, PMID:35189042
• It forms the MutL alpha heterodimer with MLH1, which is recruited to mismatched DNA by MutS complexes and executes endonucleolytic cleavage to initiate strand-specific repair.
• The endonuclease activity introduces single-strand breaks near the mismatch, providing entry points for exonuclease EXO1; strand discrimination is mediated by transient DNA methylation.
• PMS2 also participates in DNA damage signaling pathways that can induce cell cycle arrest or apoptosis in response to severe genomic lesions.
• Although PMS2 possesses ATP-binding and ATPase activities, ATP hydrolysis may not be strictly required for proficient mismatch repair under certain conditions.
• Predominantly localized in the nucleus, PMS2 functions as a tumor suppressor, and inherited mutations in the PMS2 gene are associated with hereditary nonpolyposis colorectal cancer (Lynch syndrome).
• The protein undergoes alternative splicing and is phosphorylated, yielding multiple isoforms with potentially distinct roles.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 1–100 of human PMS2, targeting the N-terminal region; confirm epitope accessibility in your assay of interest.
• For Western blotting, a predominant band around 96 kDa is expected; however, alternative splicing variants and post-translational modifications may cause additional bands or slight size shifts.
• In ELISA applications, direct detection of denatured or peptide antigens is feasible; for native protein capture, consider using a sandwich pair with an antibody recognizing a distinct epitope.
• Cross-reactivity is documented for human PMS2; if testing other species, verify the sequence homology of the N-terminal immunogen region to avoid false negatives.
• As a rabbit monoclonal antibody, it offers high reproducibility and specificity; perform initial titration and optimization experiments to determine optimal working dilutions for your sample type and detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, recombinant proteins, and bulk ancillary reagents to support diagnostic development from early research to commercial production. For DNA repair and oncology biomarker assays, our portfolio includes high-quality monoclonal antibodies like PMS2, alongside custom and rare-target sourcing solutions. Let our team streamline your supply chain so you can focus on accelerating assay development.