PMPCB Rabbit Polyclonal Antibody for Western Blot and ELISA - O75439

Core Product Specifications and Parameters

Biological Background: PMPCB Function and Localization

• PMPCB encodes the catalytic beta subunit of mitochondrial processing peptidase (MPP), a heterodimeric metalloprotease complex that resides in the mitochondrial matrix.
• MPP cleaves N-terminal mitochondrial targeting sequences from nuclear-encoded precursor proteins upon their import into mitochondria, enabling proper folding and function of the mature proteins. Related references: PMID:29576218
• Cleavage by PMPCB typically occurs after an arginine residue at the P2 position of the presequence, demonstrating arginine-specific substrate recognition.
• PMPCB plays a critical role in the turnover of PINK1, a kinase linked to Parkinson’s disease; it couples PINK1 mitochondrial import and cleavage, directing PINK1 toward proteolytic degradation. Related references: PMID:22354088
• The protein is annotated as a zinc-dependent metalloprotease and hydrolase, with direct protein sequencing evidence and identification via proteomics.
• Disease-associated variants in PMPCB are linked to neurodegenerative disorders, consistent with its mitochondrial function and keywords such as neurodegeneration and disease variant.
• Alternative names for PMPCB include MAS1, MPPB, P-52, MPP11, MPPP52, and Beta-MPP.
• Its calculated molecular weight is approximately 54 kDa (UniProt: 54366 Da), consistent with the observed protein weight in western blot.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 110–380 of human PMPCB (NP_004270.2), a region within the mature protein. Consider using mitochondrial fractions or whole-cell lysates from human, mouse, or rat cells for optimal detection.
• In Western blot, a predominant band around 54 kDa is expected. Validate the antibody in your sample system by including a positive control (e.g., HeLa or HEK293T cell lysate) and a molecular weight marker.
• For ELISA applications, titrate the antibody and use appropriate blocking agents to minimize nonspecific binding. Pair with a validated coating antigen or capture antibody as needed.
• Because this is a polyclonal antibody, lot-to-lot variability may occur. It is advisable to confirm reactivity and specificity upon receipt of each new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target procurement. This PMPCB antibody supports research into mitochondrial protein import and neurodegenerative disease mechanisms. For your diagnostic or research program, CamelBio provides end‑to‑end supply solutions from concept through clinical application.