PKM1-specific Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14618

Core Product Specifications and Parameters

Biological Background: PKM1 (Pyruvate Kinase M1) Function and Localization

• PKM1 is an isoform of pyruvate kinase generated by alternative splicing of the PKM gene, predominantly expressed in adult tissues (PubMed:18337823). Related references: PMID:18337823
• It catalyzes the final rate-limiting step of glycolysis, transferring a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, yielding ATP (PubMed:15996096, PubMed:1854723, PubMed:20847263). Related references: PMID:15996096, PMID:1854723, PMID:20847263
• PKM1 exists as a constitutively active tetramer and does not require allosteric activation by fructose-1,6-bisphosphate (FBP), in contrast to the embryonic M2 isoform (PubMed:20847263). Related references: PMID:20847263
• Subcellular localization is primarily cytoplasmic, consistent with its role in glycolysis.
• PKM1 is expressed in most adult differentiated tissues, including muscle and brain, and is notably absent or downregulated in many tumor cells, where M2 is predominant (PubMed:18337823). Related references: PMID:18337823
• The protein undergoes various post-translational modifications, including acetylation, phosphorylation, and methylation, which may regulate its activity and stability.
• PKM1 plays a critical role in energy metabolism by determining the fate of glucose carbons toward ATP production rather than biosynthetic pathways.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the C-terminal region (aa 350–450) of human PKM1, ensuring isoform specificity. Validate specificity by comparing PKM1 and PKM2 expression in control lysates.
• For Western blotting, use adult human, mouse, or rat tissue lysates (e.g., skeletal muscle, brain) as positive controls. Optimize antibody dilution based on sample type.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), anticipate cytoplasmic staining. Include appropriate antigen retrieval and blocking steps.
• Polyclonal antibodies may exhibit batch-to-batch variability; consider lot-specific validation for critical experiments.
• Cross-reactivity with mouse and rat allows use in common model organisms, but confirm sequence homology of the immunogen region if additional species are tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and academic institutes with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This PKM1-specific rabbit polyclonal antibody is part of our glycolysis and metabolism research collection. For bulk orders, custom formulations, or technical assistance, please reach out to our team.