PKM1-specific Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P14618

Core Product Specifications and Parameters

Biological Background: PKM1 Function and Localization

• PKM encodes pyruvate kinase muscle isozyme, a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP in the final rate-limiting step of glycolysis. Related references: PMID:15996096 PMID:1854723 PMID:20847263

• PKM1 is the adult-specific isoform that replaces the embryonic M2 isoform after birth. It has high pyruvate kinase activity intrinsically and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP). Related references: PMID:18337823 PMID:20847263

• PKM1 is predominantly expressed in normal adult tissues, such as muscle and brain, and is typically absent from tumor cells where PKM2 is upregulated. Related references: PMID:18337823

• The PKM protein localizes to both the cytoplasm and nucleus; however, PKM1 primarily functions in the cytoplasm as a glycolytic enzyme.

• PKM1 is subject to various post-translational modifications, including acetylation, methylation, and phosphorylation, which may modulate its activity and cellular functions.

• The PKM gene is also known by alternative names such as CTHBP (cytosolic thyroid hormone-binding protein), OIP3 (opa-interacting protein 3), and THBP1 (thyroid hormone-binding protein 1).

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide spanning residues 350–450 of human PKM1, ensuring isoform specificity. For Western blot (WB), a band of approximately 58 kDa is expected; optimize blocking and antibody dilution for the specific sample type.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) should be tested; antibody dilution and incubation conditions may need optimization based on tissue and fixation.
• In ELISA, this antibody can be used for quantitative detection of PKM1; consider pairing with a suitable detection antibody or direct conjugation for sandwich ELISA formats.
• The antibody is reported to cross-react with mouse and rat PKM1; however, validation in the target species and experimental system is recommended.
• As a rabbit monoclonal antibody, this product offers high specificity and reproducibility; include appropriate positive and negative controls in all assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and research laboratories from concept to clinic. For projects involving metabolism, glycolysis, and cancer research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This PKM1-specific antibody exemplifies our commitment to providing high-quality reagents to accelerate your assay development and research.