PKC delta Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q05655

Core Product Specifications and Parameters

Biological Background: PKC delta Function and Localization

• PKC delta (nPKC-delta) is a calcium-independent, phospholipid- and diacylglycerol-dependent serine/threonine kinase encoded by PRKCD, belonging to the protein kinase C (PKC) family.
• Functions as both pro-apoptotic and anti-apoptotic protein: promotes DNA damage-induced apoptosis via BCLAF1/p53 and ABL1 pathways, while also supporting cancer cell survival through AKT1, NF-kappa-B, and MAPK1/3 signaling. Related references: PMID:21406692 PMID:21810427 PMID:15774464
• Regulates reactive oxygen species production by phosphorylating NCF1/p47-phox in neutrophils, and modulates platelet aggregation, filopodia formation, and dense granule secretion. Related references: PMID:19801500 PMID:11748588 PMID:16940418 PMID:19587372
• Localizes to the cytoplasm, perinuclear region, nucleus, cell membrane, mitochondrion, and endomembrane system, enabling diverse signaling functions.
• Phosphorylates numerous substrates including 14-3-3 proteins, ELAVL1, PLSCR3, SMPD1, and GLI1, and is itself regulated by phosphorylation, with zinc-finger and ATP-binding domains. Related references: PMID:18285462 PMID:12649167 PMID:17303575 PMID:19015273
• Highly expressed in multiple cancers and linked to resistance to chemotherapeutic agents; mutations in PRKCD are associated with ALPS3 and CVID9.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1–160 of human PKC delta, which may result in polyclonal antibodies that recognize multiple epitopes.
• For western blot (WB), expect a band near 78 kDa; consider using reducing conditions and validate in human, mouse, or rat cell lysates.
• For IHC-P and IF/ICC, perform appropriate fixation and permeabilization steps; optimize antibody dilution empirically, starting with 1:50–1:200.
• As a polyclonal antibody, assess lot-to-lot consistency if used in quantitative assays; include knockdown or knockout controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions for diagnostic manufacturers and research laboratories. For critical targets like PKC delta, involved in apoptosis and cancer signaling, we offer validated polyclonal antibodies, bulk reagents, and custom sourcing services to support your assay development—from concept to clinic.