PKC-beta Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05771

Core Product Specifications and Parameters

Biological Background: PKC-beta Function and Localization

• Protein kinase C beta type (PKC-beta) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase encoded by the PRKCB gene. It plays pivotal roles in diverse cellular processes.
• In B-cell receptor (BCR) signaling, PKC-beta mediates canonical NF-kappa-B activation by phosphorylating CARD11/CARMA1, and provides negative feedback by phosphorylating BTK to modulate its localization and activity. Related references: PMID:11598012
• PKC-beta contributes to oxidative stress-induced apoptosis by phosphorylating isoform p66Shc of SHC1, leading to mitochondrial reactive oxygen species production.
• It acts as a coactivator of androgen receptor (AR)-dependent transcription by phosphorylating histone H3 at Thr-6, a mark that prevents H3K4 demethylation. Related references: PMID:20228790
• In insulin signaling, PKC-beta regulates glucose transport and DNA synthesis; it phosphorylates SLC2A1/GLUT1 to promote glucose uptake and modulates insulin-stimulated GLUT4 translocation in adipocytes. Related references: PMID:25982116
• PKC-beta phosphorylates ATF2 to promote transcriptional cooperation with JUN, and modulates KLHL3-WNK4 interaction in response to angiotensin II. Related references: PMID:19176525, PMID:25313067
• Recently, PKC-beta was identified as an activator of ferroptosis by phosphorylating ACSL4 and FOXK1, thereby promoting lipid peroxidation and ferroptosis propagation. Related references: PMID:35027735, PMID:40246981
• Localization is observed in the cytoplasm, nucleus, and membrane. Functional keywords include immunity, apoptosis, chromatin regulation, transcription, and metal binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 220–340 of human PKC-beta, a region within the kinase domain. Consider verifying specificity using knockout or knockdown models in the relevant species.
• For Western blot, the predicted molecular weight is approximately 77 kDa; always validate the band size in cell lines or tissues with confirmed PKC-beta expression, and account for possible post-translational modifications or isoforms.
• In immunofluorescence/ICC, PKC-beta may exhibit cytoplasmic, nuclear, or membrane localization depending on cell type and activation state. Co-stain with compartment-specific markers and include appropriate controls.
• ELISA performance should be validated in the relevant sample matrix (e.g., serum, cell lysate) using standardized protocols and a suitable detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare target sourcing—supporting projects from concept to clinic. This PKC-beta polyclonal antibody is suitable for studies in B-cell biology, oncology, endocrinology, and redox signaling, addressing key needs in IVD assay development. As a dedicated B2B partner, we integrate rigorous raw-material supply with technical consulting to accelerate your workflow while maintaining quality and consistency.