PIWIL4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q7Z3Z4

Core Product Specifications and Parameters

Biological Background: PIWIL4 Function and Localization

• PIWIL4, also known as HIWI2 or MIWI2, is a member of the Piwi protein family and exhibits RNA-binding activity essential for piRNA-mediated gene silencing.
• It plays a central role in spermatogenesis by repressing transposable elements through the piRNA metabolic process, ensuring germline integrity.
• PIWIL4 is a key component of the ping-pong amplification cycle, acting as a 'slicer-incompetent' partner that loads secondary piRNAs to target transposon loci in the nucleus.
• In the nucleus, PIWIL4-piRNA complexes mediate silencing by inducing 'Lys-9' methylation of histone H3 at specific genomic loci (PubMed:17544373). Related references: PMID:17544373
• Beyond the germline, PIWIL4 contributes to somatic cell functions, including pancreatic beta cell insulin secretion and maintaining retinal epithelial integrity via Akt/GSK3α/β signaling (PubMed:28025795). Related references: PMID:28025795
• Overexpression of PIWIL4 has been associated with oncogenic activity, inhibiting apoptosis and promoting cell proliferation in tumors (PubMed:22483988). Related references: PMID:22483988
• PIWIL4 localizes to both the nucleus and cytoplasm and is ubiquitously expressed across tissues, with notable detection in retina and retinal pigment epithelia (PubMed:17544373, 22483988, 25038252, 28025795, 28711973). Related references: PMID:17544373 PMID:22483988 PMID:25038252 PMID:28025795 PMID:28711973

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 260-460 of human PIWIL4, a region expected to be conserved across human, mouse, and rat; consider sequence alignment to confirm epitope conservation in your target species.
• For Western blotting, a band near 97 kDa is anticipated; validate detection in testis lysates or PIWIL4-overexpressing cell lines as positive controls.
• In immunohistochemistry (IHC-P), consider testis or retinal tissue sections where PIWIL4 expression is documented; optimize antigen retrieval and antibody dilution empirically.
• ELISA applications may benefit from using recombinant PIWIL4 protein as a standard; include appropriate negative controls to assess background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories comprehensive access to high-quality IVD raw materials, including validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents. For targets like PIWIL4 involved in germ cell biology and transposon silencing, we can source rare raw materials and support your assay development from concept to clinic. Contact our team to streamline your procurement and accelerate your diagnostic projects.