PIWIL4 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q7Z3Z4

Core Product Specifications and Parameters

Biological Background: PIWIL4 Function and Localization

• PIWIL4 (also designated HIWI2 or MIWI2) is a Piwi-like protein and member of the Argonaute family, central to piRNA-mediated gene silencing.
• Essential for spermatogenesis by repressing transposable elements and maintaining germline integrity via the piRNA pathway (By similarity).
• Functions in the nucleus to silence active transposons by engaging nascent transposable element transcripts and directing DNA methylation and repression (By similarity).
• Lacks endonuclease activity, acting as a 'slicer-incompetent' component in the piRNA amplification (ping-pong) loop, loading cleaved piRNAs from PIWIL2 to target genomic transposon loci (By similarity).
• Induces 'Lys-9' methylation of histone H3 at some loci, contributing to epigenetic silencing. Related references: PMID:17544373
• Ubiquitously expressed in human tissues, with detection in retina, retinal pigment epithelia and various cell types. Related references: PMID:17544373 PMID:22483988 PMID:25038252 PMID:28025795 PMID:28711973
• In somatic cells, localizes to nucleus and cytoplasm; maintains retinal epithelial integrity via Akt/GSK3alpha/beta signaling, and overexpression promotes oncogenic proliferation. Related references: PMID:28025795 PMID:22483988

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 272-383 of human PIWIL4, a region likely conserved across human, mouse, and rat based on reported cross-reactivity; consider using this antibody to detect PIWIL4 in these species.
• For Western blot (WB), a band around 97kDa is expected; however, actual migration may vary due to post-translational modifications or isoform variants. Include appropriate positive control lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate both nuclear and cytoplasmic staining consistent with PIWIL4's subcellular distribution; optimize fixation and permeabilization conditions.
• For ELISA, use recombinant PIWIL4 protein (or the immunogen) as a standard to establish assay parameters; note that the antibody may recognize denatured and native protein forms as it was raised against a recombinant fusion protein.
• Validate antibody specificity in the relevant sample system (e.g., using knockdown or knockout controls) to confirm target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your assay development by delivering validated PIWIL4 antibodies and related reagents for piRNA and germline research applications. As a one-stop IVD sourcing partner, we provide optimized monoclonal antibodies, antibody pairs, and bulk ancillary materials, plus access to rare-target raw materials to accelerate your diagnostic development from concept to clinic.