PIP5K1A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q99755

Core Product Specifications and Parameters

Biological Background: PIP5K1A Function and Localization

• PIP5K1A encodes the 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha (PIP5KIalpha), a lipid kinase central to phosphoinositide signaling.
• Catalyzes the conversion of phosphatidylinositol 4-phosphate (PI4P) to phosphatidylinositol 4,5-bisphosphate (PIP2), a critical lipid second messenger. This activity is the predominant pathway for PIP2 synthesis.
  Related references: PMID:21477596, PMID:22942276, PMID:8955136
• PIP2 serves as a precursor for the second messengers inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG), and phosphatidylinositol-3,4,5-trisphosphate (PIP3), thereby mediating diverse signal transduction cascades.
  Related references: PMID:19158393, PMID:20660631
• Through PIP2 production, PIP5K1A regulates actin cytoskeleton dynamics, cell adhesion, motility, membrane ruffling, and focal adhesion formation. It is required for phagocytosis, platelet adhesion, keratinocyte differentiation, and insulin-induced glucose transporter translocation.
• In the nucleus, PIP5K1A activates the adenylyltransferase activity of TUT1 in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs.
  Related references: PMID:18288197
• Localizes to the cell membrane, cytoplasm, nucleus, nuclear speckles, and cell projections such as ruffles and lamellipodia, reflecting its multifaceted functions.
• Highly expressed in heart, placenta, skeletal muscle, kidney, and pancreas, with lower levels in brain, lung, and liver.
• Listed as a phosphoprotein and isopeptide bond-containing protein with ubiquitin-like conjugation sites; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 231‑500 of human PIP5K1A, a region likely to contain conserved epitopes; cross-reactivity with mouse is expected and supported by sequence homology in this segment. Consider sequence alignment to confirm reactivity with other species.
• For Western blotting, a band near 63 kDa is anticipated. Use lysates from tissues with high PIP5K1A expression (e.g., heart, skeletal muscle) as positive controls, and include molecular weight markers to verify band size.
• In immunohistochemistry (IHC-P), membrane, cytoplasmic, and nuclear staining patterns may be observed depending on cell type and context. Optimize antigen retrieval and include relevant negative controls (e.g., no primary antibody or pre-immune serum) to assess specificity.
• ELISA can be employed for antibody titration or antigen detection. Validate the assay using recombinant PIP5K1A protein or cell lysates, and determine optimal coating and detection conditions.
• As a polyclonal antibody, it may detect multiple isoforms or degradation products; confirm specificity by knockdown or overexpression experiments in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PIP5K1A antibody supports research into lipid signaling, cytoskeletal dynamics, and cell migration, and is part of our validated portfolio of polyclonal antibodies. Beyond individual antibodies, we offer optimized antibody pairs, bulk ancillary reagents, and sourcing for rare targets, streamlining your development workflow with reliable supply and expertise.