PIK3CG Rabbit Polyclonal Antibody for WB, ELISA - P48736

Core Product Specifications and Parameters

Biological Background: PIK3CG Function and Localization

• PIK3CG encodes the p110γ catalytic subunit of class I phosphoinositide 3-kinase (PI3K), also known as PI3Kγ, a lipid kinase that phosphorylates PtdIns(4,5)P2 to generate PIP3.
• PIP3 serves as a membrane anchor for PH domain-containing proteins such as AKT1 and PDPK1, triggering signaling pathways that regulate cell growth, survival, proliferation, and motility.
• p110γ links G protein-coupled receptor (GPCR) activation to PIP3 production, playing a crucial role in immune, inflammatory, and allergic responses.
• The protein is critical for leukocyte chemotaxis and migration by controlling PIP3 spatial accumulation, F-actin organization, and integrin-based adhesion at the leading edge.
• PIK3CG also possesses serine/threonine protein kinase activity, which together with its lipid kinase activity is required for GPCR endocytosis and regulation of cardiac contractility.
• It is expressed in pancreas, skeletal muscle, liver, and heart, and localizes to the cytoplasm and cell membrane.
• The protein is a negative regulator of cardiac contractility and contributes to pathological cardiac hypertrophy through kinase-dependent and scaffolding functions.
• Post-translational modifications and scaffolding interactions enable PIK3CG to assemble signaling complexes involved in angiogenesis, platelet aggregation, and neutrophil responses.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-310) of human PIK3CG; this may allow detection of full-length and potentially truncated forms.
• For Western blot, expect a band near 126 kDa; validate in human tissues or cell lines known to express PIK3CG, such as leukocytes, heart, or skeletal muscle.
• In ELISA, consider optimizing the coating antigen and antibody dilution using recombinant protein or cell lysates.
• As a polyclonal antibody, performance may vary between lots; re-validate each new lot in the relevant assay system.
• Cross-reactivity with species other than human has not been characterized; for non-human samples, perform sequence alignment and experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For immunology and cardiovascular research, our anti-PIK3CG antibody supports studies on GPCR signaling and immune cell migration. We specialize in rare-target raw-material sourcing, helping you accelerate development from concept to clinic.