PIAS3 Rabbit pAb for WB, IHC-P, ELISA - Q9Y6X2

Core Product Specifications and Parameters

Biological Background: PIAS3 Function and Localization

• PIAS3 (Protein Inhibitor of Activated STAT3) is an E3 SUMO-protein ligase and SUMO-tethering factor that stabilizes the interaction between the E2 enzyme UBE2I and substrates to catalyze SUMO conjugation. Related references: PMID:21965678, PMID:9388184
• It functions as a transcriptional coregulator in multiple signaling pathways, notably inhibiting STAT3 DNA-binding activity and suppressing STAT3-mediated cell growth.
• PIAS3 enhances the sumoylation of metastasis-associated protein MTA1 and may contribute to paralog-selective sumoylation of this protein. Related references: PMID:21965678
• It sumoylates CCAR2 (DBC1), which facilitates the interaction between CCAR2 and the deacetylase SIRT1, connecting SUMO modification to deacetylation regulatory networks. Related references: PMID:25406032
• PIAS3 can diminish sumoylation of ZFHX3 (ATBF1) by preventing its colocalization with SUMO1 in the nucleus, thereby modulating the transcriptional repressor function of ZFHX3. Related references: PMID:24651376
• The protein localizes predominantly to the cytoplasm, nucleus, and nuclear speckles, consistent with its roles in subnuclear signaling and transcription regulation.
• PIAS3 is widely expressed across human tissues and contains a zinc-finger domain essential for its ligase activity and substrate recognition.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (a.a. 452–628) of human PIAS3; the antibody may recognize multiple isoforms or post-translationally modified forms. Consider verifying specificity for your isoform of interest via sequence alignment and epitope mapping if cross-reactivity with PIAS family members is a concern.
• For Western blotting, the predicted molecular weight is approximately 68 kDa; however, sumoylated, phosphorylated, or other post-translationally modified species may yield additional bands at higher or aberrant migration. Validate in your sample system using appropriate positive and negative controls.
• In immunohistochemistry (paraffin), antigen retrieval methods such as heat-induced epitope retrieval (HIER) may be required for optimal detection. Given the predicted cytoplasmic and nuclear localization, confirm staining patterns with known expression data.
• For ELISA, the recombinant immunogen or full-length PIAS3 protein can be used as a capture antigen. Optimize coating concentrations and assay conditions, and include appropriate control antigens and secondary antibody controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated procurement partner for IVD antibody development, supplying researchers and manufacturers with rigorously selected raw materials from concept to clinical application. For studies on PIAS3 and STAT-mediated transcriptional regulation, we offer validated polyclonal antibodies, matched antibody pairs, stabilizing buffers, and ancillary reagents in bulk quantities. Contact us for custom sourcing of rare-target antibodies or one-stop technical support tailored to your diagnostic pipeline.