PIAS2 Rabbit Monoclonal Antibody for WB, ELISA - O75928

Core Product Specifications and Parameters

Biological Background: PIAS2 Function and Localization

• Functions as an E3 SUMO-protein ligase and transcriptional coregulator in the STAT, p53, and steroid hormone signaling pathways; involves both transactivation and gene silencing, primarily silencing.
• Isoform PIAS2-beta, but not PIAS2-alpha, promotes MDM2 sumoylation; isoform PIAS2-alpha promotes PARK7 sumoylation and sumoylates PML at Lys-65 and Lys-160.
• Binds sumoylated ELK1 and enhances its transcriptional activity by preventing HDAC2 recruitment, reversing SUMO-mediated repression.
• Localizes to nuclear speckles, PML bodies, and the nucleoplasm.
• Mainly expressed in testis, with isoform 3 predominantly in adult testis; weak expression in pancreas and sperm.
• Contains zinc-finger domains and DNA-binding capacity; undergoes alternative splicing and phosphorylation.
• Keywords: Alternative splicing, DNA-binding, Isopeptide bond, Metal-binding, Nucleus, Phosphoprotein, Transferase, Ubl conjugation pathway, Zinc.

Experimental Guidance and Technical Tips

• For Western blot, optimize primary antibody concentration; use molecular weight markers to detect bands at 45, 63, or 68 kDa corresponding to different isoforms.
• In ELISA applications, ensure proper antigen coating and validate using recombinant target proteins or cell lysates.
• Verify cross-reactivity with mouse and rat samples as indicated; include appropriate species-specific controls.
• Consider siRNA knockdown or isoform-specific controls to confirm specificity when studying individual PIAS2 isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions comprehensive access to IVD raw materials, including this PIAS2 monoclonal antibody for SUMOylation and transcriptional regulation studies. Our supply capabilities cover validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development. We also provide technical services and consulting to support your project from concept to clinic.