PIAS1 Rabbit Polyclonal Antibody for WB and ELISA - O75925

Core Product Specifications and Parameters

Biological Background: PIAS1 Function and Localization

• PIAS1, also named GBP, ZMIZ3, DDXBP1, or GU/RH-II, is an E3 SUMO-protein ligase and SUMO-tethering factor that catalyzes the covalent attachment of SUMO to substrates such as CEBPB, MRE11, PML, and ZNF76. Related references: PMID:11583632 PMID:11867732 PMID:14500712
• It functions as a transcriptional coregulator in multiple signaling pathways, including the STAT, p53, and steroid hormone pathways, where it can either activate or repress gene expression depending on the biological context. Related references: PMID:11583632 PMID:11867732
• In vitro, PIAS1 binds A/T-rich DNA, suggesting a direct role in chromatin targeting or transcriptional regulation. Related references: PMID:15133049
• PIAS1 mediates sumoylation of MRE11, which stabilizes MRE11 on chromatin during DNA end resection, linking it to DNA damage repair. Related references: PMID:36050397
• It enhances the sumoylation of the metastasis-associated protein MTA1 and may participate in its paralog-selective sumoylation. Related references: PMID:21965678
• PIAS1 restricts lytic replication of Epstein-Barr virus (EBV) by inhibiting transcription factors required for lytic gene expression, though the virus can use caspases to antagonize this restriction. Related references: PMID:29262325
• Subcellularly, PIAS1 localizes predominantly to the nucleus, including nuclear speckles and PML bodies, and is also found associated with the cytoskeleton.
• PIAS1 is ubiquitously expressed but shows highest expression in testis, as indicated by tissue profiling.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–140) of human PIAS1; consider sequence alignment with mouse and rat orthologs to assess cross-reactivity for your specific experiments.
• For Western blot (WB), a band at approximately 72 kDa is expected. Validate the antibody in your cell or tissue lysate system, as post-translational modifications or isoforms may affect migration.
• In ELISA applications, consider using a recombinant protein covering the immunogen region as a positive control to confirm binding specificity.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is recommended to include appropriate positive and negative controls and to optimize the working dilution for each new batch.
• Since PIAS1 is predominantly nuclear, using nuclear-enriched lysates for WB may improve detection sensitivity.
• For quantitative WB analysis, normalize using total protein staining (e.g., stain-free technology) or a validated housekeeping protein to ensure accurate loading comparisons.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a comprehensive one-stop sourcing platform for diagnostic manufacturers, laboratories, and research institutes, offering a complete range of IVD raw materials and services from early development to clinical application. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents, with a special focus on rare and emerging targets like PIAS1 in the field of transcriptional regulation and SUMOylation. By bridging research and supply, CamelBio helps you secure the critical raw materials needed for robust assay development and efficient scaling.