PIAS1 Rabbit Polyclonal Antibody for IF/ICC and ELISA - O75925

Core Product Specifications and Parameters

Biological Background: PIAS1 Function and Localization

• PIAS1 (Protein Inhibitor of Activated STAT1) is an E3 SUMO-protein ligase also known as GBP, ZMIZ3, DDXBP1, and GU/RH-II. It mediates the sumoylation of diverse substrates through its SP-RING domain, functioning as both a SUMO ligase and SUMO-tethering factor. Related references: PMID:11583632 PMID:11867732 PMID:14500712

• As a transcriptional coregulator, PIAS1 modulates the STAT, p53, and steroid hormone signaling pathways, with transactivation or silencing effects that depend on the biological context. Related references: PMID:11583632 PMID:11867732

• PIAS1 sumoylates key proteins including CEBPB, MRE11, MTA1, PTK2, PML, and ZNF76, regulating processes such as DNA repair, adipogenesis, and transcriptional repression. Related references: PMID:11583632 PMID:11867732 PMID:14500712 PMID:21965678 PMID:36050397

• It binds A/T-rich DNA in vitro and is capable of recognizing the TAAT box in gene promoters, contributing to its role in chromatin association and gene regulation. Related references: PMID:15133049

• PIAS1 localizes predominantly to the nucleus, including nuclear speckles and PML bodies, but can also be found in the cytoplasm and associated with the cytoskeleton, reflecting its diverse functional roles.

• Tissue expression is broad, with the highest level observed in testis, suggesting specialized functions in spermatogenesis or testicular gene regulation.

• The protein contains a zinc-finger domain and is subject to acetylation, phosphorylation, and alternative splicing, as indicated by UniProt keywords such as Zinc, Zinc-finger, Acetylation, Phosphoprotein, and Alternative splicing.

• In host-virus interactions, PIAS1 restricts Epstein-Barr virus lytic replication by inhibiting transcription factors required for lytic gene expression, a function that can be counteracted by viral caspases. Related references: PMID:29262325

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 422–651 of human PIAS1, covering the C-terminal region that includes the SP-RING domain critical for SUMO ligase activity. The antibody may recognize both native and SUMOylated forms; optimize fixation and permeabilization for IF/ICC to preserve nuclear and cytoplasmic epitopes.
• This antibody is validated for Indirect Immunofluorescence (IF/ICC) and ELISA. For other applications such as Western blotting (expected molecular weight ~72 kDa), validate specificity and sensitivity with appropriate positive and negative controls.
• The antibody is raised against human PIAS1 and shows cross-reactivity with human protein. For use in other species, check sequence homology in the immunogen region (residues 422-651) and validate experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing of IVD raw materials, from validated antibodies to bulk ancillary reagents, supporting diagnostic manufacturers and researchers from concept to clinic. Our PIAS1 polyclonal antibody serves the SUMOylation and transcriptional regulation research community, complementing our portfolio of high-quality immunological reagents. Whether you need custom antibody development, optimized antibody pairs, or rare target sourcing, CamelBio streamlines your supply chain with reliable, one-stop access.