PI3 Kinase p85 alpha Rabbit pAb - P27986

Core Product Specifications and Parameters

Biological Background: PI3 Kinase p85 alpha Function and Localization

• Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3K p85 alpha) is the 85 kDa regulatory subunit of class IA PI3Ks, encoded by the PIK3R1 gene. It is also known as p85, GRB1, and p85alpha.
• Acts as an adapter protein that binds to activated phosphorylated protein-tyrosine kinases via its SH2 domain, mediating the association of the p110 catalytic subunit to the plasma membrane. This interaction is necessary for insulin-stimulated glucose uptake and glycogen synthesis, and plays a role in signaling through FGFR, KIT, PDGFRA/B, and ITGB2. Related references: PMID:17626883, PMID:19805105, PMID:7518429
• Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in an ER stress- and/or insulin-dependent manner, contributing to improved glucose tolerance in the liver. Related references: PMID:20348923
• Predominantly localizes to the cytoplasm.
• Isoform 2 is expressed in skeletal muscle and brain, with lower levels in kidney and cardiac muscle; isoforms 2 and 4 are detected at the protein level in skeletal muscle.
• Contains SH2 and SH3 domains, and undergoes post-translational modifications including acetylation and phosphorylation. Associated with disease variants linked to immunodeficiency 36 (IMD36) and plays roles in host-virus interaction, protein transport, and stress response.

Experimental Guidance and Technical Tips

• The immunogen (aa 240-380) lies within a region that may include functional SH2 domains; consider epitope accessibility in native versus denatured sample preparations.
• For Western blot, the predicted molecular weight is 84 kDa; use appropriate gel percentage and transfer conditions. Validate the detected band size in your system.
• Cross-reactivity with mouse and rat extends the utility to common model organisms, but confirm specificity in your experimental setup.
• For IHC-P and IF/ICC, test antigen retrieval methods and antibody dilutions, as fixation may affect epitope availability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop access to IVD raw materials, supporting your PI3K signaling and metabolic research with validated antibody pairs, optimized polyclonal antibodies like this anti-PI3 Kinase p85 alpha, and bulk ancillary reagents. Our expertise extends to sourcing rare-target raw materials, helping you bridge from concept to clinical application efficiently.