PHYHD1 Rabbit pAb for WB, IF/ICC, ELISA - Q5SRE7

Core Product Specifications and Parameters

Biological Background: PHYHD1 Function and Localization

• PHYHD1 is a phytanoyl-CoA dioxygenase domain-containing protein 1, acting as a 2-oxoglutarate (2OG)-dependent dioxygenase that converts 2OG to succinate and CO₂ in an iron-dependent manner. Related references: PMID:21530488
• It does not couple 2OG turnover to the hydroxylation of acyl-coenzyme A derivatives; no activity is detected towards fatty acid CoA thioesters, indicating it is not directly involved in phytanoyl-CoA metabolism. Related references: PMID:21530488
• Alternative splicing produces isoforms 2 and 3, which probably lack catalytic enzyme activity.
• PHYHD1 contains a conserved iron-binding site typical of the 2OG-dependent dioxygenase family, classifying it as an oxidoreductase acting on single donors with incorporation of molecular oxygen.
• Phosphorylation of PHYHD1 has been identified through proteomics, suggesting regulatory post-translational modification.
• A 3D structure has been determined for PHYHD1, offering insights into its active site and catalytic mechanism.
• It is annotated as a reference proteome component, ensuring high-quality functional and structural annotation.

Experimental Guidance and Technical Tips

• The antibody, raised against full-length recombinant PHYHD1 (aa 1–291), is expected to detect a band near 32 kDa in WB using lysates from human, mouse, or rat cells.
• For IF/ICC, optimize fixation and permeabilization conditions for your specific cell type; consider that PHYHD1 may localize to specific subcellular compartments not yet defined.
• Since the immunogen covers the entire protein, the antibody likely recognizes all isoforms; confirm isoform-specific expression with additional sequence- or domain-specific tools if needed.
• Because PHYHD1 activity depends on iron, preserve metal-binding conditions if functional assays are planned downstream.
• ELISA performance can be further validated for quantitative detection; consider pairing with a capture antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For research and assay development involving PHYHD1 and related 2OG dioxygenases, this polyclonal antibody provides a reliable tool, and CamelBio can support further sourcing needs—from rare target reagents to custom production—to help streamline your path from concept to clinic.