Phospho-ZAP70-Y493 Rabbit mAb - P43403

Core Product Specifications and Parameters

Biological Background: ZAP70 Function and Localization

• ZAP70 (Tyrosine-protein kinase ZAP-70), also known as Syk-related tyrosine kinase, is a 70 kDa cytoplasmic protein belonging to the Syk kinase family.
• It plays a pivotal role in adaptive immunity by regulating T-cell and B-cell receptor signaling, affecting motility, adhesion, cytokine expression, and thymocyte development.
• Upon TCR engagement, ZAP70 is recruited to the phosphorylated ITAM motifs of CD3E and CD247/CD3Z at the plasma membrane, which relieves its autoinhibition and leads to activation (PubMed:7509083). Related references: PMID:7509083
• Activated ZAP70 phosphorylates key adaptor proteins LAT and LCP2, assembling signaling complexes that drive lymphokine production, T-cell proliferation, and differentiation.
• ZAP70 also modulates T-cell cytoskeleton dynamics, adhesion, and mobility, and participates in TCR downregulation by promoting CD247/CD3Z internalization and degradation via CBL and adaptor proteins SLA/SLA2.
• Subcellular localization is mainly cytoplasmic and at the cell membrane, with expression restricted to T-cells, natural killer cells, early thymocytes, and pro/pre B-cells.
• Post-translational modifications include phosphorylation (critical for activity), acetylation, and ubiquitination; the protein features SH2 domains, an ATP-binding kinase domain, and multiple phosphorylation sites.

Experimental Guidance and Technical Tips

• The antibody targets the phosphorylation site at Tyr493, a critical residue for ZAP70 activation; consider using phosphatase inhibitors during sample preparation to preserve the phospho-epitope.
• For IF/ICC, validate staining specificity by using a non-phosphorylated peptide block or treating cells with a phosphatase to confirm signal loss.
• This antibody is suitable for ELISA; when adapting to other immunoassays, titrate and test in your specific sample matrix to ensure linearity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing—all under one roof. This phospho-specific ZAP70 antibody supports adaptive immunity research, particularly in T-cell signaling and immunological disorder studies. Reach out to explore how our end-to-end supply chain can accelerate your diagnostic development from concept to clinic.