Phospho-YWHAZ (Ser58)/YWHAH (Ser59)/YWHAG (Ser59)/YWHAB (Ser60) Rabbit Monoclonal Antibody for WB, ELISA - P63104 / Q04917 / P61981 / P31946

Core Product Specifications and Parameters

Biological Background: 14-3-3 protein zeta/delta Function and Localization

• Adapter protein involved in regulating a broad range of general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438).
  Related references: PMID:14578935, PMID:15071501, PMID:15644438
• Binds to numerous protein partners primarily through recognition of phosphorylated serine or threonine motifs, often modulating their activity (PubMed:35662396).
  Related references: PMID:35662396
• Promotes cytoplasmic retention and inactivation of TFEB transcription factor by binding to its phosphorylated form (PubMed:35662396).
  Related references: PMID:35662396
• Induces ARHGEF7 activity on RAC1, leading to lamellipodia and membrane ruffle formation (PubMed:16959763).
  Related references: PMID:16959763
• In neurons, regulates spine maturation through modulation of ARHGEF7 activity (By similarity).
• Localizes primarily to the cytoplasm and is also found in melanosomes.
• Undergoes post-translational modifications including phosphorylation and acetylation.
• Disease-associated variants have been linked to intellectual disability.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phospho-peptide immunogen; ensure sample phosphorylation status is preserved during preparation (e.g., use phosphatase inhibitors).
• For WB applications, consider validating signal specificity by treating lysates with lambda phosphatase or comparing with non-phospho-specific antibodies.
• In ELISA, use the phospho-peptide as positive control and non-phospho peptide as negative control to confirm phospho-specificity.
• Given the cross-reactivity with multiple 14-3-3 family members, confirm isoform-specific detection by employing siRNA knockdown or recombinant protein controls if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing by offering a comprehensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents to diagnostic manufacturers and research laboratories. For phospho-specific detection and 14-3-3 signaling research, we provide reliable access to rare-target antibodies and bulk reagent supplies. Partner with us to bridge the gap from concept to clinic with efficient, end-to-end procurement solutions.