phospho-YAP1 (S397) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P46937

Core Product Specifications and Parameters

Biological Background: YAP1 Function and Localization

• YAP1 (Yes-associated protein 1, aliases YAP65, YAP, YAP2, YAP-1, YKI, COB1) is a transcriptional coactivator and corepressor critical for Hippo pathway-mediated organ size control and tumor suppression. It promotes proliferation inhibition and apoptosis. Related references: PMID:17974916 | PMID:18280240 | PMID:18579750
• In the canonical Hippo kinase cascade, MST1/2 and LATS1/2 phosphorylate YAP1 at multiple residues including S397, preventing its nuclear translocation and coactivator function. Related references: PMID:18158288 | PMID:26598551 | PMID:34404733
• YAP1 interacts with TEAD transcription factors to drive expression of target genes governing cell growth, anchorage-independent proliferation, and epithelial-mesenchymal transition (EMT). Related references: PMID:18579750
• Through ARHGAP18, YAP1 modulates the cortical actomyosin network, influencing tissue tension and 3D tissue architecture. Related references: PMID:25778702
• YAP1 can suppress ciliogenesis by repressing AURKA and PLK1 expression via TEAD4. Related references: PMID:25849865
• Subcellularly, YAP1 localizes to the cytoplasm, nucleus, tight junctions, and cell membrane; its shuttling is controlled by phosphorylation-dependent 14-3-3 binding.
• As a proto-oncogene, YAP1 is overexpressed in certain liver and prostate cancers; specific isoforms are found in Huntington disease striatal neurons.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide encompassing the phosphorylated S397 residue of human YAP1, ensuring site-specificity. Validation may include phosphatase-treated lysates or phospho-blocking peptides in Western blot.
• For Western blotting, a band at ~54 kDa is expected; verify endogenous expression levels in relevant cell lysates and optimize antibody dilutions empirically.
• YAP1 is widely expressed; confirm expression in your experimental system using appropriate positive and negative controls.
• The antibody shows cross-reactivity with human, mouse, and rat; for other species, consider sequence alignment of the phosphorylated epitope.
• Optimal conditions for IF/ICC and ELISA should be individually determined; consider including no-primary and peptide competition controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, including this phospho-YAP1 (S397) antibody for Hippo pathway and oncology research. Our offerings encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare target raw materials to accelerate your diagnostic assay development from concept to clinic.