Phospho-Vimentin-S56 Rabbit pAb for WB, ELISA - P08670

Core Product Specifications and Parameters

Biological Background: Vimentin Function and Localization

• Vimentin is a type III intermediate filament protein encoded by the VIM gene, predominantly expressed in mesenchymal cells and widely used as an epithelial-mesenchymal transition (EMT) marker.
• Vimentin filaments connect the nucleus, endoplasmic reticulum, and mitochondria, playing a key role in maintaining cytoarchitecture and facilitating organelle positioning during directional cell migration.
• Vimentin interacts with LARP6 to stabilize type I collagen mRNAs (COL1A1 and COL1A2), contributing to extracellular matrix production and tissue fibrosis.
• The protein undergoes extensive post-translational modifications including phosphorylation, acetylation, and ubiquitination; phosphorylation at Ser-56 (the target of this antibody) is implicated in regulating filament assembly/disassembly dynamics.
• Subcellular localization is primarily cytoplasmic and cytoskeletal, with additional pools in the nuclear matrix, cell membrane, and neuronal axons, reflecting its diverse functional roles.
• Tissue expression is high in fibroblasts and certain immune cells, with variable levels in cancer cell lines; vimentin is frequently upregulated in metastatic and mesenchymal-derived tumors.

Experimental Guidance and Technical Tips

• Always include phosphatase inhibitors in lysis buffers during sample preparation to preserve the S56 phosphorylation signal.
• Validate antibody specificity by treating samples with λ‑phosphatase or using phosphatase inhibitor‑positive controls; loss of signal upon dephosphorylation confirms phospho‑specificity.
• For Western blot, run a reducing SDS‑PAGE gel and transfer to PVDF or nitrocellulose membrane; the expected band is ~54 kDa (may shift slightly depending on phosphorylation status).
• This antibody is also suitable for ELISA; optimize coating conditions and detection reagents according to your specific assay format.
• Cross-reactivity is reported for human and rat; if using other species, perform in‑house validation due to potential sequence variations around the phosphorylation site.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD manufacturers, providing a comprehensive portfolio of raw materials including phospho‑specific antibodies like Anti-Phospho‑Vimentin‑S56 for cell migration and cancer research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for difficult-to-find raw materials—streamlining your assay development from concept to clinic.