Phospho-Vimentin-S39 Rabbit pAb for WB, IHC-P, ELISA - P08670

Core Product Specifications and Parameters

Biological Background: Vimentin Function and Localization

• Vimentin is a class-III intermediate filament protein primarily expressed in mesenchymal cells, fibroblasts, and some lymphocytes.
• It forms filamentous networks that link the nucleus, endoplasmic reticulum, and mitochondria, providing mechanical support and aiding in organelle positioning.
• Vimentin plays critical roles in cell migration, directional movement, and Golgi complex polarization at the leading edge of migrating cells.
• It stabilizes type I collagen mRNAs (CO1A1 and CO1A2) through interaction with LARP6, contributing to extracellular matrix production.
• Phosphorylation at Ser39 and other residues regulates filament disassembly and reorganization during signaling events.
• The protein is located in the cytoplasm, cytoskeleton, nucleus matrix, cell membrane, and cell projections like axons, reflecting its dynamic roles.
• Post-translational modifications include acetylation, phosphorylation, glycation, S-nitrosylation, and ubiquitination, which modulate its function and interactions.
• Mutations in VIM are associated with cataract and other disease states.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser39 of human vimentin; antibody specificity should be verified using phosphatase-treated lysates or non-phosphorylated peptide competition.
• For Western blot (WB), consider using a reducing agent and boiling samples to unfold intermediate filament proteins, but validate in your specific experimental system.
• In immunohistochemistry (IHC-P), antigen retrieval methods and fixation conditions may affect phospho-epitope accessibility; optimization is recommended.
• ELISA applications may quantify phospho-vimentin levels; include appropriate positive and negative controls.
• Cross-reactivity with mouse and rat vimentin is reported based on sequence homology, but sequence alignment of the immunogen region is advised for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials. Our phospho-vimentin antibody supports research into cell migration, mesenchymal transition, and cytoskeletal dynamics. We offer optimized antibody pairs, bulk reagents, and custom sourcing solutions to accelerate your diagnostic assay development from concept to clinic.