Phospho-VEGF Receptor 2-Y1175 Rabbit mAb for WB, ELISA - P35968

Core Product Specifications and Parameters

Biological Background: VEGF Receptor 2 Function and Localization

• VEGF Receptor 2 (VEGFR2, also known as KDR, FLK-1, CD309) is a tyrosine-protein kinase receptor that binds VEGFA, VEGFC, and VEGFD, playing an essential role in angiogenesis, vascular development, and vascular permeability.
• It promotes endothelial cell proliferation, survival, migration, and differentiation, as well as actin cytoskeleton reorganization.
• Isoforms lacking the transmembrane domain (e.g., isoform 2) act as decoy receptors, negatively regulating lymphangiogenesis by limiting free VEGFA/VEGFC.
• Upon ligand binding, it activates multiple signaling cascades, including PLCG1, MAPK/ERK, AKT1, and PI3K pathways, and mediates NOS2/NOS3 induction for nitric oxide production.
• Subcellular localization includes cell membrane, endoplasmic reticulum, cytoplasm, nucleus, cytoplasmic vesicles, early endosomes, and secreted forms.
• It is widely expressed, with detection in cornea, and is classified under keywords such as angiogenesis, differentiation, kinase, receptor, and phosphoprotein.
• The protein has a molecular weight of approximately 152 kDa (151.5 kDa based on UniProt).

Experimental Guidance and Technical Tips

• This phospho-specific antibody targets phosphorylation at tyrosine 1175 (Y1175) of human VEGFR2. Verify phosphorylation-dependent recognition by treating samples with VEGF or phosphatase inhibitors.
• For Western blot, consider using appropriate positive controls (e.g., VEGF-stimulated endothelial cells) to confirm detection.
• For ELISA, validate the antibody’s performance with phosphorylated peptide versus non-phosphorylated control to ensure specificity.
• Always validate the antibody in your specific sample system and application, as conditions may influence performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Whether you need validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio’s integrated supply chain can support your angiogenesis and VEGFR2-related research and diagnostic development. Leverage our expertise to streamline your procurement and accelerate your project timelines.