Phospho-VAV1-Y174 Rabbit pAb for WB - P15498

Core Product Specifications and Parameters

Biological Background: VAV1 Function and Localization

• The VAV1 proto-oncogene encodes a signal transducing protein that functions as a guanine nucleotide exchange factor (GEF), coupling tyrosine kinase signals with the activation of Rho/Rac GTPases to regulate cell differentiation and/or proliferation.
• The protein contains SH2 and SH3 domains along with a zinc-finger motif, indicating roles in phosphotyrosine-mediated protein interactions and metal coordination.
• Alternative splicing generates multiple isoforms (UniProt annotation), though specific variants are not characterized in this entry.
• Widely expressed in hematopoietic cells but not in other cell types, underscoring its specialized role in blood and immune cell signaling.
• As a phosphoprotein, its activity is subject to tight regulation by tyrosine phosphorylation; Y174 is one of the critical phosphorylation sites.
• Classified as a proto-oncogene, dysregulation of VAV1 is associated with oncogenic transformation in hematopoietic malignancies.

Experimental Guidance and Technical Tips

• For phospho-specific detection, consider using a phosphopeptide blocking experiment to confirm specificity.
• Validate the antibody in human and rat hematopoietic cell lines that express VAV1.
• Prepare samples with phosphatase inhibitors to preserve phosphorylation status; the predicted molecular weight is approximately 98 kDa, though shifts may be observed due to phosphorylation.
• Optimize primary antibody dilution for WB; typical starting conditions can be explored using lysates from Jurkat or other T-cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with seamless access to a comprehensive range of IVD raw materials, technical services, and consulting—from concept to clinic. For projects focused on hematopoietic signaling, oncology, or phospho-specific antibody workflows, CamelBio supports your pipeline with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our sourcing model ensures timely delivery and quality consistency, empowering your development of robust immunoassays and diagnostic tools.