Phospho-VANGL2-T78/S79/S82 Rabbit mAb for WB and ELISA - Q9ULK5

Core Product Specifications and Parameters

Biological Background: VANGL2 Function and Localization

• VANGL2 (Vang-like protein 2), also known as Loop-tail protein 1 homolog, Strabismus 1, or Van Gogh-like protein 2, is a transmembrane protein encoded by the VANGL2 gene (GeneID: 57216).
• It plays a critical role in planar cell polarity (PCP) signaling, regulating early morphogenesis, patterning of axial midline structures, and neural plate development.
• VANGL2 is essential for the polarization and movement of myocardializing cells in the cardiac outflow tract, acting via RHOA signaling.
• In the inner ear, VANGL2 is required for the orientation of stereociliary bundles and for the cell surface localization of the Wnt receptors FZD3 and FZD6.
• The protein localizes to the cell membrane and contains four transmembrane helices.
• Post-translational modifications include phosphorylation at multiple residues, including T78, S79, and S82, which may regulate its activity in PCP signaling.
• Keywords associated with VANGL2 include developmental protein, disease variant, and transmembrane protein.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic phospho-specific peptide corresponding to residues surrounding T78/S79/S82 of human VANGL2. This suggests the antibody specifically recognizes the phosphorylated form of VANGL2 at these sites.
• For WB, we recommend detecting the target band at approximately 60 kDa. Species cross-reactivity includes human, mouse, and rat; consider using appropriate positive control lysates from these species.
• For ELISA, titrate the antibody against the phospho-peptide and a non-phospho control to confirm phosphorylation-specific binding.
• Since VANGL2 is a membrane protein, ensure adequate solubilization during sample preparation.
• Validate the antibody in the relevant cellular context, such as cells with activated planar cell polarity signaling.

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