Phospho-β-Catenin-Y333 Rabbit pAb for WB - P35222

Core Product Specifications and Parameters

Biological Background: β-Catenin Function and Localization

• Key downstream component of the canonical Wnt signaling pathway. In the presence of Wnt, β-catenin accumulates and translocates to the nucleus, where it acts as a coactivator for TCF/LEF transcription factors to activate Wnt-responsive genes. Related references: PMID:17524503 PMID:18077326 PMID:18086858
• In the absence of Wnt, β-catenin is phosphorylated by a destruction complex (AXIN, APC, GSK3B) and targeted for ubiquitination and proteasomal degradation, ensuring low cytoplasmic levels. Related references: PMID:17524503 PMID:18077326 PMID:18086858
• Functions in cell adhesion as part of the E‑cadherin/catenin adhesion complex, linking cadherins to the actin cytoskeleton at adherens junctions.
• Localizes to multiple subcellular sites including cytoplasm, nucleus, cell junctions, centrosome, spindle poles, and synapse, reflecting its diverse roles in signaling and structural organization.
• Expressed in hair follicle cells, colon epithelium, cortical neurons, and breast cancer tissues (at protein level). Related references: PMID:29367600
• Involved in embryonic development, EMT, neurogenesis, and centrosome regulation; dysregulation is linked to cancer and neurodevelopmental disorders.

Experimental Guidance and Technical Tips

• The immunogen is a phospho‑specific peptide corresponding to residues around Tyr333; consider treating cell/tissue lysates with phosphatase inhibitors to preserve the phosphorylation state.
• For Western blot, the predicted band size is ~85 kDa. Include appropriate molecular weight markers and verify band specificity using appropriate controls (e.g., peptide competition, phosphatase treatment, or stimulation/inhibition of upstream kinases).
• Optimize blocking, primary antibody dilution, and detection conditions for your specific sample type. Non‑specific bands may appear; confirm identity via siRNA knockdown or other independent methods.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, from validated antibodies to ancillary reagents. For Wnt signaling and cancer‑focused projects, this phospho‑specific β‑Catenin antibody complements our broad selection of monoclonal and polyclonal antibodies, bulk antigens, and rare‑target sourcing options. Let us streamline your procurement from concept to clinic with reliable, one‑stop supply solutions.