Phospho-β-Catenin-S675 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P35222

Core Product Specifications and Parameters

Biological Background: Beta-catenin Function and Localization

• Key downstream component of the canonical Wnt signaling pathway: In the absence of Wnt, β-catenin is phosphorylated and degraded; upon Wnt stimulation, it accumulates in the nucleus, coactivating TCF/LEF transcription factors to drive target gene expression.
  Related references: PMID:17524503, PMID:18077326, PMID:18086858
• Functions in cell adhesion as part of the E-cadherin:catenin complex at adherens junctions.
• Acts as a negative regulator of centrosome cohesion (PubMed:18086858).
  Related references: PMID:18086858
• Promotes epithelial to mesenchymal transition (EMT) and mesenchymal to epithelial transition (MET) by driving CTNNB1/TCF target genes (PubMed:29910125).
  Related references: PMID:29910125
• Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353).
  Related references: PMID:21262353
• Blocks anoikis of malignant kidney and intestinal epithelial cells, promoting anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423).
  Related references: PMID:18957423
• Disrupts PML function and PML nuclear body formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184).
  Related references: PMID:22155184
• Localizes to multiple subcellular compartments: cytoplasm, nucleus, adherens junctions, centrosomes, spindle poles, synapses, and cilium basal body. Expressed in hair follicle cells, colon, cortical neurons, and breast cancer tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide matching residues around S675 of human β‑catenin; antibody recognition is likely specific for the phosphorylated form. Use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blot, the predicted molecular weight is approximately 85 kDa; consider a 6–10% SDS-PAGE gel and appropriate transfer conditions.
• This antibody has been applied in WB, IF‑P, and ELISA. Validate each application with appropriate positive and negative controls (e.g., phosphatase‑treated lysates).
• The reported cross‑reactivity with human, mouse, and rat samples makes it suitable for studies in common model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated B2B partner for IVD raw‑material procurement, supporting diagnostic manufacturers and research teams from concept to commercialization. This Phospho‑β‑Catenin‑S675 rabbit polyclonal antibody exemplifies our offering for Wnt signaling and cancer research applications—our portfolio extends to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need a single component or a complete raw‑material package, CamelBio streamlines your sourcing to accelerate assay development.