Phospho-β-Catenin-S552 Rabbit Monoclonal Antibody for WB, ELISA - P35222

Core Product Specifications and Parameters

Biological Background: Phospho-β-Catenin (Ser552) Function and Localization

• Catenin beta-1 (β-catenin) is a dual-function protein acting both in cell adhesion and in Wnt signal transduction. It links E-cadherin to the actin cytoskeleton at adherens junctions and shuttles to the nucleus to activate TCF/LEF target genes.

• In the absence of Wnt ligand, β-catenin is phosphorylated on N-terminal Ser/Thr residues, ubiquitinated by the β-TrCP E3 ligase, and degraded by the proteasome. Upon Wnt stimulation, β-catenin is stabilized and translocates to the nucleus (PubMed:17524503, PubMed:18077326, PubMed:18086858).

  Related references: PMID:17524503 PMID:18077326 PMID:18086858

• Nuclear β-catenin acts as a coactivator for TCF/LEF transcription factors and also cooperates with other factors such as NR5A2 (PubMed:22187462) to regulate target gene expression.

  Related references: PMID:22187462

• β-catenin drives epithelial-mesenchymal transition (EMT) by inducing expression of mesenchymal genes (PubMed:29910125).

  Related references: PMID:29910125

• It localizes to multiple subcellular compartments including the cytoplasm, nucleus, and membrane–cytoskeleton interfaces such as adherens junctions, centrosomes, spindle poles, and the cilium basal body.

• β-catenin is expressed in various tissues including hair follicle cells, colon epithelium, cortical neurons, and breast cancer tissues (PubMed:29367600).

  Related references: PMID:29367600

• Post-translational modifications include N-terminal phosphorylation, ubiquitination, acetylation, S-nitrosylation, and sumoylation, which modulate its stability and activity.

Experimental Guidance and Technical Tips

• For Western blot, prepare lysates from cells stimulated with Wnt ligands or treated with proteasome inhibitors to enrich for phosphorylated β-catenin and reduce degradation.
• The immunogen is a synthetic phospho-peptide corresponding to Ser552; therefore, the antibody may recognize both the phosphorylated and non-phosphorylated forms, but specificity can be confirmed by comparing signals from phosphatase-treated samples.
• As the antibody cross-reacts with human and mouse, it is suitable for analyzing β-catenin phosphorylation in these species; validate in the relevant tissue or cell system.
• The expected molecular weight for β-catenin is approximately 85 kDa; use an appropriate molecular weight marker and gel percentage for clear separation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop supply chain for IVD raw materials, from concept to clinic. This phospho-β-catenin (Ser552) antibody is ideal for Wnt signaling and cell adhesion research. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development.