Phospho-ULK1-S467 Rabbit pAb for WB, ELISA - O75385

Core Product Specifications and Parameters

Biological Background: ULK1 Function and Localization

• ULK1 is a serine/threonine-protein kinase essential for autophagy initiation, particularly in response to nutrient starvation. Related references: PMID:18936157, PMID:21460634, PMID:21795849
• It acts upstream of the phosphatidylinositol 3-kinase PIK3C3 to regulate autophagophore formation, a key early step in autophagy. Related references: PMID:18936157, PMID:21460634, PMID:21795849
• ULK1 is part of a feedback loop with mTORC1, acting both as a downstream effector and a negative regulator of mTORC1 signaling via interaction with RPTOR. Related references: PMID:21795849
• AMPK phosphorylates and activates ULK1, while ULK1 in turn phosphorylates AMPK subunits to negatively regulate AMPK activity. Related references: PMID:21460634
• ULK1 phosphorylates multiple autophagy-related substrates, including FLCN, AMBRA1, ATG4B, SESN2, and SQSTM1, thereby modulating autophagosome nucleation, maturation, and inhibition. Related references: PMID:25126726, PMID:20921139, PMID:28821708, PMID:25040165, PMID:37306101
• In neuronal development, ULK1 is required for granule cell axon formation and plays a role early in neuronal differentiation. Related references: PMID:11146101
• ULK1 localizes to the cytoplasm, cytosol, and preautophagosomal structures where it orchestrates autophagosome formation.
• The protein is ubiquitously expressed, with detection in skeletal muscle, heart, brain, placenta, liver, kidney, lung, and pancreas.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide around serine 467 of human ULK1; consider using phosphatase inhibitors during sample preparation to preserve phosphorylation signals.
• For ELISA, this phospho-specific antibody may require optimization of peptide coating or blocking conditions to achieve specific detection.
• Given ULK1's role in autophagy, validate phosphorylation changes in starved versus nutrient-rich conditions to confirm pathway activation.
• Cross-reactivity is reported for human and mouse; for other species, check the homology of the S467 flanking sequence before use.
• The predicted molecular weight of ULK1 is approximately 113 kDa; a band near this size is expected, though post-translational modifications may cause slight shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials for autophagy and kinase research, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing bridges the gap from concept to clinic, supporting diagnostic manufacturers and research labs with reliable materials and technical consultation. This phospho-ULK1 (S467) antibody is part of our extensive catalog for studying autophagy-related pathways in disease.