Phospho-Tyk2 (Y1054/1055) Rabbit Polyclonal Antibody for WB and ELISA - P29597

Core Product Specifications and Parameters

Biological Background: TYK2 Function and Localization

• Non-receptor tyrosine-protein kinase involved in numerous cytokines and interferons signaling, regulating cell growth, development, migration, innate and adaptive immunity. Related references: PMID:10542297, PMID:10995743, PMID:7657660
• Plays both structural and catalytic roles in interleukins and interferons (IFN-alpha/beta) signaling. Related references: PMID:10542297
• Associates with heterodimeric cytokine receptor complexes (e.g., IFNAR1, IL12RB1, IL10RB, IL13RA1) and activates STAT family members (STAT1, STAT3, STAT4, STAT6). Related references: PMID:10542297, PMID:7638186, PMID:25762719, PMID:7526154
• Upon cytokine binding, phosphorylates receptor chains, creating docking sites for STATs; recruited STATs are then phosphorylated, form dimers, and translocate to the nucleus to regulate gene expression. Related references: PMID:10542297, PMID:25762719, PMID:7526154, PMID:7657660
• Negatively modulates STAT3 activity by promoting phosphorylation at a distinct tyrosine residue, dampening signaling output. Related references: PMID:29162862
• Functions in oncostatin-M-mediated signaling via both type I (LIFR/IL6ST) and type II (OSMR/IL6ST) receptor complexes. Related references: PMID:9188471
• Expressed in all cell lines analyzed, in both lymphoid and non-lymphoid cells.
• Keywords include ATP-binding, kinase, nucleotide-binding, phosphoprotein, SH2 domain, transferase, and tyrosine-protein kinase; undergoes phosphorylation at Y1054/1055 residues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y1054 and Y1055; include phosphatase inhibitors during sample preparation to preserve phosphorylation status.
• For Western blotting, the predicted molecular weight is ~134 kDa; optimize protein transfer and loading conditions for large proteins.
• The antibody cross-reacts with mouse and rat Tyk2; validate in your species of interest.
• For ELISA, carefully optimize coating antigen and detection conditions to achieve desired sensitivity and specificity.
• Consider confirming specificity with non-phosphorylated peptide blockade if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This phospho-specific Tyk2 antibody supports the development of assays targeting the JAK-STAT signaling pathway, expanding your capabilities in cytokine, kinase, and immunology research. With validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and rare target sourcing, we are your partner from concept to clinic.