Phospho-TBC1D4-S588 Rabbit pAb for WB, ELISA - O60343

Core Product Specifications and Parameters

Biological Background: TBC1D4 Function and Localization

• TBC1D4, also known as AS160 (Akt substrate of 160 kDa), is a multidomain protein that functions as a GTPase-activating protein (GAP) for Rab proteins, including RAB2A, RAB8A, RAB10, and RAB14.
• Isoform 2 of TBC1D4 plays a critical role in insulin-stimulated GLUT4 translocation to the plasma membrane, thereby increasing glucose uptake in adipocytes and skeletal muscle cells.
• TBC1D4 is a phosphoprotein with multiple phosphorylation sites; phosphorylation at Ser588 is associated with insulin signaling and regulates its GAP activity and interaction with 14-3-3 proteins.
• The protein is widely expressed, with isoform 1 highly expressed in skeletal muscle and heart, while isoform 2 is abundant in adrenal gland, thyroid, lung, kidney, colon, brain, and adipose tissue.
• In pancreatic islets, TBC1D4 expression is decreased in type 2 diabetes patients, linking it to the pathophysiology of diabetes mellitus.
• Subcellularly, TBC1D4 localizes primarily to the cytoplasm, where it interacts with vesicles and signaling components.
• Additional post-translational modifications include acetylation and methylation, which may further modulate its function.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using whole-cell lysates from insulin-stimulated versus unstimulated cells to validate phosphorylation-dependent detection; include phosphatase inhibitors during sample preparation.
• Due to the high molecular weight (~147 kDa), use an appropriate gel percentage and transfer conditions to ensure efficient detection; verify the band size with a molecular weight marker.
• In ELISA, the antibody can be utilized for phospho-peptide detection; include non-phosphorylated peptide controls to confirm specificity.
• The antibody cross-reacts with human, mouse, and rat TBC1D4; validate in the relevant species and tissue context, keeping in mind the tissue-specific expression profiles.
• As a polyclonal antibody from rabbit, lot-to-lot consistency may vary; consider re-validation when switching to a new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing for diagnostic manufacturers and research laboratories by offering a comprehensive catalog of validated antibodies and ancillary reagents. This Phospho-TBC1D4-S588 Rabbit pAb supports metabolic and diabetic research, and we can source related antibody pairs, bulk monoclonal/polyclonal antibodies, and other rare target reagents to accelerate your assay development. Contact us to discuss custom sourcing of phosphorylated-antibody panels and consistent supply for your clinical biomarker projects.