Phospho-Tau-T217 Rabbit mAb for WB, ELISA - P10636-8

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau is a microtubule-associated protein encoded by the MAPT gene, primarily expressed in neurons where it stabilizes microtubules and promotes axonal transport.
• Alternative splicing generates six major tau isoforms in the adult human brain; Isoform Tau-F (P10636-8) is one of these variants, differing in N-terminal inserts and microtubule-binding repeats.
• Tau hyperphosphorylation leads to its dissociation from microtubules and aggregation into paired helical filaments (PHFs), forming neurofibrillary tangles—a hallmark of Alzheimer's disease and other tauopathies.
• The Thr217 phosphorylation site is among the early and prominent pathological modifications, contributing to tau dysfunction and aggregation in neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide specific to the T217 phosphorylation site; pre-absorption with the non-phosphorylated peptide is recommended to confirm phospho-specificity in Western blot.
• For Western blot, consider using a total tau antibody as a loading control to verify equal protein loading and assess phosphorylation levels.
• The predicted molecular weight of isoform Tau-F is ~46 kDa, but post-translational modifications, alternative splicing, and aggregation can result in additional bands; validate in the relevant cell or tissue lysates.
• In ELISA, use the antibody in combination with a capture antibody for tau to measure phospho-T217 tau levels; optimize coating and blocking conditions for human samples.
• Always freshly prepare lysates with phosphatase inhibitors to preserve phosphorylation status; run positive and negative controls to validate signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated phospho-Tau (Thr217) antibodies and matched pairs. Our portfolio supports Alzheimer's and neurodegenerative disease assay development with bulk antibodies, recombinant proteins, and ancillary reagents. Leverage our sourcing expertise to secure customized monoclonal antibodies and rare target materials for your next-generation diagnostic kits.